A urinary pentasaccharide in bovine mannosidosis.
A urinary pentasaccharide in bovine mannosidosis.
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牛甘露糖苷沉积症中的尿五糖。
DOI：
10.1111/j.1432-1033.1975.tb02488.x
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
R. Jolly
中科院分区：
文献类型：
--
作者：
A. Lundblad;B. Nilsson;N. Nordén;S. Svensson;P. Ockerman;R. Jolly
Abnormally high amounts of low molecular weight mannose-rich carbohydrate material were found in the urine of an Angus calf with mannosidosis. At least five oligosaccharide fractions were detected by paper chromatography. The most abundant compound was purified by gel chromatography, zone electrophoresis, and two consecutive preparative paper chromatographic steps. The yield was 10 mg/liter of urine. From structural studies including nuclear magnetic resonance spectroscopy, optical rotation, sugar analysis, methylation analysis, and partial enzymatic degradation the following structure was deduced: alpha-D-Manp-(1 leads to 6)-beta-D-Manp-(1 leads to 4)-beta-D-GlcNAcp-(1 leads to 4)-beta-D-GlcNAcp-(1 leads to 4)-D-GlcNAc. This oligosaccharide is distinct from all the oligosaccharides previously described which are excreted by patients with mannosidosis.
