Continued selection on cryptic SARS-CoV-2 observed in Missouri wastewater.
Continued selection on cryptic SARS-CoV-2 observed in Missouri wastewater.
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DOI：
10.1371/journal.ppat.1011688
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发表时间：
2023-12
期刊：
影响因子：
6.7
通讯作者：
中科院分区：
文献类型：
--
作者：
在2019冠状病毒病大流行期间，对废水进行深度测序以检测SARS-CoV-2，以监测病毒变体在社区中出现和传播的情况。在临床样本中未检测到的来源不明的SARS-CoV-2谱系，称为隐性谱系，有时会在特定地点重复检测到。我们继续检测到一个这样的血统以前看到在密苏里州网站。这种神秘的血统继续进化，表明持续的选择压力类似于Omicron血统中观察到的。监测废水中的SARS-CoV-2是了解大流行期间病毒在社区内和社区间传播和持续存在的动态的重要组成部分。我们和其他人还观察到废水中出现的变异体在临床采样中没有出现。这些变异体中的许多不仅具有与在关注的变异体（特别是目前流行的Omicron变异体）中观察到的基因组变化相同或在相同位置处的基因组变化，而且通常在临床样品中观察到这些变化之前获得这些变化。我们在这里报告了在密苏里州废水中持续观察到的变体，但不是在临床采样中，该变体继续进化，获得的基因组变化通常是相同的，并且早于临床样本中观察到的变化。这些观察增加了我们对驱动SARS-CoV-2进化的选择性压力的理解。
Deep sequencing of wastewater to detect SARS-CoV-2 has been used during the COVID-19 pandemic to monitor viral variants as they appear and circulate in communities. SARS-CoV-2 lineages of an unknown source that have not been detected in clinical samples, referred to as cryptic lineages, are sometimes repeatedly detected from specific locations. We have continued to detect one such lineage previously seen in a Missouri site. This cryptic lineage has continued to evolve, indicating continued selective pressure similar to that observed in Omicron lineages. Monitoring wastewater for SARS-CoV-2 has been an important part of understanding the dynamics of the virus’s spread and persistence within and across communities during the pandemic. We and others have also observed variants appearing in wastewater that do not appear in clinical sampling. Many of these variants not only possess genomic changes identical to or at the same position as those that have been observed in variants of concern, particularly currently circulating Omicron variants, but often acquire the changes before they have been observed in clinical samples. We report here the continued observation of a variant in Missouri wastewater, but not in clinical sampling, that has continued to evolve, gaining genomic changes that often are the same and predate changes seen in clinical samples. These observations add to our understanding of the selective pressures driving the evolution of SARS-CoV-2.
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影响因子：
6.7
作者：
通讯作者：
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影响因子：
6.7
作者：
通讯作者：
--
DOI：
10.1126/science.abf9302
发表时间：
2021-02-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Starr TN;Greaney AJ;Addetia A;Hannon WW;Choudhary MC;Dingens AS;Li JZ;Bloom JD
通讯作者：
Bloom JD
影响因子：
3.7
作者：
Trujillo M;Cheung K;Gao A;Hoxie I;Kannoly S;Kubota N;San KM;Smyth DS;Dennehy JJ
通讯作者：
Dennehy JJ
影响因子：
5.4
作者：
Johnson MC;Lyddon TD;Suarez R;Salcedo B;LePique M;Graham M;Ricana C;Robinson C;Ritter DG
通讯作者：
Ritter DG