AMINO-ACID-SEQUENCE HOMOLOGY IN GAG REGION OF REVERSE TRANSCRIBING ELEMENTS AND THE COAT PROTEIN GENE OF CAULIFLOWER MOSAIC-VIRUS
AMINO-ACID-SEQUENCE HOMOLOGY IN GAG REGION OF REVERSE TRANSCRIBING ELEMENTS AND THE COAT PROTEIN GENE OF CAULIFLOWER MOSAIC-VIRUS
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DOI：
10.1093/nar/14.2.623
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发表时间：
1986-01-24
影响因子：
14.9
通讯作者：
COVEY, SN
中科院分区：
文献类型：
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作者：
COVEY, SN
一种源自逆转录病毒 gag 基因的核酸结合蛋白 (NBP)，被认为与病毒粒子核心中的基因组 RNA 相互作用，含有高度保守的半胱氨酸残基排列。对可用核酸和蛋白质序列的搜索表明，基序 CysX2CysX4HisX4Cys (NBPcys) 在所有具有复制能力的逆转录病毒、叙利亚仓鼠脑池内 A 粒子基因、果蝇逆转录转座子基因和花椰菜花叶病毒 (CaMV) 中都是不变的。在每种情况下，NBPcys 都位于“gag-pol”区域中保守蛋白酶氨基酸序列之前的部分。这对于 CaMV 具有特殊意义，因为 NBPcys 位于推定逆转录酶基因 (ORF V) 上游的外壳蛋白基因 (ORF IV) 中，并表明逆转录元件的 gag 极性排列在 CaMV 中得以保留。此外，CaMV 与所有其他已知的含有 NBPcys 的元件不同，因为它将 DNA 基因组包装在病毒体中。
A nucleic acid binding protein (NBP) derived from thegaggene of retroviruses that is thought to interact with genomic RNA in virion cores, contains a highly conserved arrangement of cysteine residues. A search of available nucleic acid and protein sequences has revealed that the motif CysX2CysX4HisX4Cys (NBPcys) is invarlent in all replication competent retroviruses, a Syrian hamster intracisternal A-particle gene, theDrosophilaretrotransposoncopiaand in cauliflower mosaic virus (CaMV). In each case, NBPcys is located in that part of the ‘gag-pol’ region Just preceding a conserved protease amino acid sequence. This is of special significance for CaMV as NBPcys is in the coat protein gene (ORF IV) upstream of the putative reverse transcrlptase gene (ORF V) and demonstrates that thegag-polarrangement of reverse transcribing elements is preserved in CaMV. Moreover, CaMV differs from all other known NBPcys-con-taining elements in that it packages a DNA genome in virions.