Antibacterial activity within degradation products of biological scaffolds composed of extracellular matrix
Antibacterial activity within degradation products of biological scaffolds composed of extracellular matrix
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DOI：
10.1089/ten.2006.12.2949
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发表时间：
2006-10-01
影响因子：
--
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
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作者：
Brennan, Ellen P.;Reing, Janet;Badylak, Stephen F.
由细胞外基质（ECM）组成的生物支架在临床前体内研究中已显示出对故意细菌污染的抗性。本研究评价了ECM支架酸消化产生的降解产物对金黄色葡萄球菌和大肠杆菌临床菌株的抗菌作用。ECM支架来源于猪膀胱（UBM-ECM）和肝脏（L-ECM）。这些生物支架在高温下用酸消化，使用硫酸铵沉淀分级，并在标准化的体外测定中测试抗菌活性。UBM-ECM和L-ECM的降解产物均表现出对S. aureus和E.杆菌显示抗微生物活性的特定硫酸铵级分对于2种不同的ECM支架类型而变化。这项研究的结果表明，几种不同的低分子量的抗菌活性肽存在于ECM和这些肽可能有助于解释这种生物支架提供的细菌感染的阻力。
Biological scaffolds composed of extracellular matrix (ECM) have been shown to be resistant to deliberate bacterial contamination in preclinical in vivo studies. The present study evaluated the degradation products resulting from the acid digestion of ECM scaffolds for antibacterial effects against clinical strains of Staphylococcus aureus and Escherichia coli. The ECM scaffolds were derived from porcine urinary bladder (UBM-ECM) and liver (L-ECM). These biological scaffolds were digested with acid at high temperatures, fractionated using ammonium sulfate precipitation, and tested for antibacterial activity in a standardized in vitro assay. Degradation products from both UBM-ECM and L-ECM demonstrated antibacterial activity against both S. aureus and E. coli. Specific ammonium sulfate fractions that showed antimicrobial activity varied for the 2 different ECM scaffold types. The results of this study suggest that several different low-molecular-weight peptides with antibacterial activity exist within ECM and that these peptides may help explain the resistance to bacterial infection provided by such biological scaffolds.