The microbiota on different oral surfaces in healthy children
The microbiota on different oral surfaces in healthy children
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DOI：
10.1111/j.1399-302x.2008.00493.x
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发表时间：
2009-06-01
影响因子：
--
通讯作者：
Papagiannoulis, L.
中科院分区：
文献类型：
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作者：
Papaioannou, W.;Gizani, S.;Papagiannoulis, L.
知识的早期口腔定植模式可以提供一个更好的了解口腔生物膜的发展和疾病的启动，反过来又可以为基础的早期预防programmes.Microbial样本收集从五个不同的口腔栖息地，共93名儿童（年龄3-12岁），参加牙科学校的雅典大学，谁被分成三个年龄组。通过棋盘式DNA-DNA杂交技术共检测到38种微生物，除微小单胞菌和牙龈卟啉单胞菌外，所有检测菌种在不同采样点之间存在显著差异，为不同口腔表面提供了非常不同的微生物谱。龈上和龈下菌斑具有相似的轮廓，并表现出较高比例的放线菌属和绿色复合体，而软组织样本主要由黄色复合体的链球菌。舌背和唾液的轮廓也相似。许多物种在给定地点的所有三个年龄组中的比例相似。牙周病病原体的比例随着年龄的增长而增加。具体而言，在所有样本位置中，红色复合种（Tannerella denthia、P. gingivalis、Treponema denticola）显示出随年龄增长的比例显著增加（P < 0.05）。年龄组之间的分布差异表明口腔微生物群逐渐成熟，由越来越多的橙子和红色复合物种组成。
Knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes.Microbial samples were collected from five different oral habitats from a total of 93 children (age 3-12 years), attending the Dental School of the University of Athens, who were split into three age groups. A total of 38 microbial species were sought out by the checkerboard DNA-DNA hybridization technique.All of the test species, except Parvimonas micra and Porphyromonas gingivalis, differed significantly among sample locations providing quite distinct microbial profiles for the different oral surfaces. Supragingival and subgingival plaque had similar profiles and exhibited higher proportions of Actinomyces species and Green complex while soft tissue samples were dominated by streptococci of the Yellow complex. The profiles of the tongue dorsum and saliva were also similar. Many of the species were in similar proportions in all three age groups for a given location. Periodontal pathogens showed increases in proportions with increasing age. Specifically, the Red complex species (Tannerella forsythia, P. gingivalis, Treponema denticola) showed a significant increase in proportion with age (P < 0.05) in all sample locations.The results showed a pattern of colonization in children similar to that previously found in adults. Differences in the profile between age groups suggest a gradual maturation of the oral microbiota, with it being made up of an increasing number of Orange and Red complex species.