Genomic regions controlling vernalization and photoperiod responses in oat
Genomic regions controlling vernalization and photoperiod responses in oat
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DOI：
10.1007/s00122-001-0845-5
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发表时间：
2002-07-01
影响因子：
5.4
通讯作者：
Lee, M
中科院分区：
文献类型：
--
作者：
Holland, JB;Portyanko, VA;Lee, M
燕麦基因型因光周期和春化反应而异。春化常常促使秋播品种提早开花，而春播品种则没有。较长的光周期也促进了较早的开花，高纬度地区的品种对较长的光周期的响应往往更大。为了研究燕麦光周期和春化反应的遗传基础，我们在Ogle x TAM O-301生长室内定位群体中定位了4个光周期和春化处理组合下开花时间的qtl。我们还绘制了早春和晚春大田种植开花时间的qtl，以确定燕麦对早春种植响应的遗传基础。在大多数条件下检测到3个主要的花期qtl （OT8、OT31和OT32连锁群）。对开花影响较小的qtl在不同处理中观察到的一致性较差。春化敏感qtl和春化不敏感qtl均被发现。较长的光周期或单独春化倾向于降低花时qtl的效应。同时应用，较长的光周期和春化通常在相同的基因组区域协同作用。早春播种对种子的春化作用减弱。本研究中定位的主要开花时间qtl与之前在Kanota X Ogle燕麦定位群体中定位的qtl相匹配。在这两项研究中，我们发现了开花时间qtl的一致性，分离扭曲和复杂的遗传连锁。这些影响可能都与六倍体燕麦的染色体重排有关。燕麦与其他禾本科植物的比较定位将有助于燕麦春化反应的分子分析。
Oat genotypes vary for photoperiod and vernalization responses. Vernalization often promotes earlier flowering in fall-sown but not spring-sown cultivars. Longer photoperiods also promote earlier flowering, and the response to longer photoperiods tends to be greater in cultivars from higher latitudes. To investigate the genetic basis of photoperiod and vernalization responses in oat, we mapped QTLs for flowering time under four combinations of photoperiod and vernalization treatments in the Ogle x TAM O-301 mapping population in growth chambers. We also mapped QTLs for flowering time in early spring and late-spring field plantings to determine the genetic basis of response to early spring planting in oat. Three major flowering-time QTLs (on linkage groups OT8, OT31 and OT32) were detected in most conditions. QTLs with smaller effects on flowering were less-consistently observed among treatments. Both vernalization-sensitive and insensitive QTLs were discovered. Longer photoperiod or vernalization alone tended to decrease the effects of flowering-time QTLs. Applied together, longer photoperiod and vernalization interacted synergistically, often on the same genomic regions. Earlier spring planting conferred an attenuated vernalization treatment on seeds. The major flowering-time QTLs mapped in this study matched those mapped previously in the Kanota X Ogle oat mapping population. Between these two studies, we found a concordance of flowering-time QTLs, segregation distortion, and complex genetic linkages. These effects may all be related to chromosomal rearrangements in hexaploid oat. Comparative mapping between oat and other grasses will facilitate molecular analysis of vernalization response in oat.