Membrane proteins: always an insoluble problem?
Membrane proteins: always an insoluble problem?
复制标题
膜蛋白：始终是一个无法解决的问题？
DOI：
10.1042/bst20160025
复制
发表时间：
2016-06-15
影响因子：
3.9
通讯作者：
Rawlings AE
中科院分区：
文献类型：
--
作者：
Rawlings AE
膜蛋白在细胞过程中起着至关重要的作用，并且通常是重要的药理学药物靶点。这些蛋白质的疏水特性使得完整的结构和功能表征具有挑战性，因为当从其天然脂质膜中提取它们时需要使用洗涤剂或其他增溶剂。为了帮助膜蛋白的研究，需要新的方法，使这些蛋白质的表达和纯化便宜，容易，高产率，并提供水溶性蛋白质的后续研究。这篇迷你评论主要集中在水溶性膜蛋白的相对较新的领域，特别是两种创新的方法：重新设计膜蛋白以产生水溶性变体，以及如何添加增溶融合蛋白有助于克服这些挑战。这篇综述也着眼于天然存在的膜蛋白，它们能够以稳定的、功能性的、水溶性的组装体存在，而不改变它们的天然序列。
Membrane proteins play crucial roles in cellular processes and are often important pharmacological drug targets. The hydrophobic properties of these proteins make full structural and functional characterization challenging because of the need to use detergents or other solubilizing agents when extracting them from their native lipid membranes. To aid membrane protein research, new methodologies are required to allow these proteins to be expressed and purified cheaply, easily, in high yield and to provide water soluble proteins for subsequent study. This mini review focuses on the relatively new area of water soluble membrane proteins and in particular two innovative approaches: the redesign of membrane proteins to yield water soluble variants and how adding solubilizing fusion proteins can help to overcome these challenges. This review also looks at naturally occurring membrane proteins, which are able to exist as stable, functional, water soluble assemblies with no alteration to their native sequence.