Increased expression and role of thymic stromal lymphopoietin in nasal polyposis.
Increased expression and role of thymic stromal lymphopoietin in nasal polyposis.
复制标题
DOI：
10.4168/aair.2011.3.3.186
复制
发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Okubo K
中科院分区：
文献类型：
--
作者：
Kimura S;Pawankar R;Mori S;Nonaka M;Masuno S;Yagi T;Okubo K
鼻息肉病是一种慢性上呼吸道炎症性疾病，常与哮喘相关，其特征是嗜酸性粒细胞、Th2型淋巴细胞、成纤维细胞、杯状细胞和肥大细胞数量显著增加。先前的研究表明，在哮喘、特应性皮炎等特应性疾病中，胸腺间质淋巴生成素(TSLP)水平升高，主要是在过敏性鼻炎(AR)的动物模型中。在这里，我们研究了特应性鼻息肉和非特应性鼻息肉中TSLP的表达，并与鼻黏膜进行了比较，探讨了TSLP在鼻息肉病中的潜在作用。采用实时荧光定量聚合酶链式反应技术检测特应性鼻息肉和非特应性鼻息肉组织中TSLP、胸腺及活化调节趋化因子(TARC)和巨噬细胞衍生趋化因子(MDC)的信使RNA表达。用免疫组织化学方法检测鼻息肉和鼻黏膜组织中TSLP的免疫反应。用Wright-Giemsa染色检测嗜酸性粒细胞计数，用ELISA法检测鼻息肉组织中的IgE。鼻息肉组织中TSLP、TARC和MDC的信使RNA表达明显高于变态反应性鼻黏膜组织。鼻黏膜和鼻息肉的上皮细胞、内皮细胞、成纤维细胞和炎性细胞表达TSLP。AR患者鼻黏膜中TSLP+细胞数量明显多于NAR患者。特应性鼻息肉组织中TSLP+细胞数显著高于非特应性鼻息肉组织和变应性鼻黏膜组织。鼻息肉组织中TSLP+细胞数与嗜酸性粒细胞数、IgE水平呈正相关。TSLP在鼻息肉中的高表达及其与嗜酸性粒细胞和IgE的相关性提示，TSLP可能通过调节Th2型和嗜酸性炎症在鼻息肉的发病机制中发挥作用。
Nasal polyposis is a chronic inflammatory disease of the upper airways often associated with asthma and characterized by markedly increased numbers of eosinophils, Th2 type lymphocytes, fibroblasts, goblet cells and mast cells. Previous studies have shown elevated levels of thymic stromal lymphopoietin (TSLP) in atopic diseases like asthma, atopic dermatitis and mainly in animal models of allergic rhinitis (AR). Here, we investigated the expression of TSLP in nasal polyps from atopics and non-atopics in comparison with the nasal mucosa and its potential role in nasal polyposis. Messenger RNA expression for TSLP, thymus and activation-regulated chemokine (TARC) and macrophage derived chemokine (MDC) in nasal polyps and nasal mucosa of atopics and non-atopics was analyzed by real time PCR. Immunoreactivity for TSLP in nasal polyps and in the nasal mucosa of patients with AR and non-allergic rhinitis (NAR) was analyzed by immunohistochemistry. Eosinophil counts was analyzed by Wright-Giemsa staining and nasal polyp tissue IgE, by ELISA. Messenger RNA expression for TSLP,TARC and MDC was markedly higher in nasal polyps as compared to the allergic nasal mucosa. Immunoreactivity for TSLP was detected in epithelial cells, endothelial cells, fibroblasts and inflammatory cells of the nasal mucosa and nasal polyps. The number of TSLP+ cells was significantly greater in the nasal mucosa of AR than NAR patients. The number of TSLP+ cells in nasal polyps from atopics was significantly greater than that of non-atopics and that in the allergic nasal mucosa. The number of TSLP+ cells correlated well with the number of eosinophils and the levels of IgE in nasal polyps. The high expression of TSLP in nasal polyps and its strong correlation to eosinophils and IgE suggest a potential role for TSLP in the pathogenesis of nasal polyps by regulating the Th2 type and eosinophilic inflammation.