Novel polyelectrolyte carboxymethyl konjac glucomannan-chitosan nanoparticles for drug delivery.: II.: Release of albumin in vitro
Novel polyelectrolyte carboxymethyl konjac glucomannan-chitosan nanoparticles for drug delivery.: II.: Release of albumin in vitro
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DOI：
10.1002/jbm.b.30156
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发表时间：
2005-02-15
影响因子：
3.4
通讯作者：
Peng, YX
中科院分区：
文献类型：
--
作者：
Du, J;Zhang, S;Peng, YX
羧甲基魔芋葡甘聚糖-壳聚糖（CKGM-CS）纳米颗粒是在非常温和的条件下通过聚电解质络合自发制备的。牛血清白蛋白（BSA）作为模型蛋白药物，被掺入 CKGM-CS 纳米颗粒中。通过 Zetasizer 3000 和 FTIR 分光光度法鉴定了 BSA 负载纳米粒子的物理化学性质。它们的尺寸从330 nm到900 nm；根据不同的 CKGM/CS 比率，zeta 电位呈正值。封装效率提高20%。还研究了纳米颗粒中 BSA 的体外释放行为。我们可以发现，CKGM-CS 纳米颗粒释放的 BSA 更受 CS 涂层的影响，而不是 CKGM 内部结构的影响。 CKGM-CS 基质不仅表现出 pH 响应特性，而且还表现出离子强度敏感特性。这些系统可能具有脉冲蛋白药物输送的潜力。 (C) 2004 年 Wiley 期刊公司。
Carboxymethyl konjac glucomannan-chitosan (CKGM-CS) nanoparticles were spontaneously prepared under very mild conditions via polyelectrolyte complexation. Bovine serum albumin (BSA), as a model protein drug, was incorporated into the CKGM-CS nanoparticles. The physicochemical properties of the BSA-loaded nanoparticles were identified by Zetasizer 3000 and FTIR spectrophotometry. Their sizes were from 330 nm to 900 nm; zeta potentials were positive according to varies CKGM/CS ratios. The encapsulation efficiency was up 20%. The release behavior in vitro of BSA from the nanoparticles was also investigated. We could find that the BSA release from the CKGM-CS nanoparticles is much more influenced by the CS coating layer than by the CKGM inner structure. And the CKGM-CS matrices not only exhibited pH-responsive properties, but ionic strength-sensitive properties. These systems may present a potential for pulsatile protein drug delivery. (C) 2004 Wiley Periodicals, Inc.