Antigen recognition determinants of gammadelta T cell receptors.
Antigen recognition determinants of gammadelta T cell receptors.
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发表时间：
2005
期刊：
影响因子：
56.9
通讯作者：
Sunny Shin;R. El-Diwany;S. Schaffert;E. Adams;K. Garcia;P. Pereira;Y. Chien
中科院分区：
文献类型：
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作者：
Sunny Shin;R. El-Diwany;S. Schaffert;E. Adams;K. Garcia;P. Pereira;Y. Chien
γ δ T细胞受体（TCR）识别的分子基础知之甚少。在这里，我们分析的TCR序列的天然γ δ T细胞群体的主要组织相容性复合体Ib类分子T22。我们发现，T22识别强烈相关的体细胞重组TCR δ互补决定区3（CDR3）基序来自生殖系编码的残基。这些残基周围的序列多样性调节TCR配体结合亲和力，而V基因的使用主要与组织来源相关。这些结果显示了抗原特异性γ δ TCR库是如何以高频率产生的，并表明γ δ T细胞识别有限数量的抗原。
The molecular basis of gammadelta T cell receptor (TCR) recognition is poorly understood. Here, we analyze the TCR sequences of a natural gammadelta T cell population specific for the major histocompatibility complex class Ib molecule T22. We find that T22 recognition correlates strongly with a somatically recombined TCRdelta complementarity-determining region 3 (CDR3) motif derived from germ line-encoded residues. Sequence diversity around these residues modulates TCR ligand-binding affinities, whereas V gene usage correlates mainly with tissue origin. These results show how an antigen-specific gammadelta TCR repertoire can be generated at a high frequency and suggest that gammadelta T cells recognize a limited number of antigens.