IN-VIVO EFFECTS OF HYPERINSULINEMIA ON LIPOGENIC ENZYMES AND GLUCOSE-TRANSPORTER EXPRESSION IN RAT-LIVER AND ADIPOSE TISSUES
IN-VIVO EFFECTS OF HYPERINSULINEMIA ON LIPOGENIC ENZYMES AND GLUCOSE-TRANSPORTER EXPRESSION IN RAT-LIVER AND ADIPOSE TISSUES
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DOI：
10.1016/0026-0495(95)90270-8
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发表时间：
1995-02-01
影响因子：
9.8
通讯作者：
JEANRENAUD, B
中科院分区：
文献类型：
--
作者：
ASSIMACOPOULOSJEANNET, F;BRICHARD, S;JEANRENAUD, B
对正常大鼠施加慢性高胰岛素血症并维持正常血糖4天。在白色脂肪组织中，高胰岛素血症导致GLUT 4蛋白和mRNA增加2倍，脂肪酸合成酶（FAS）和乙酰辅酶A（CoA）羧化酶（ACC）活性分别增加6倍至15倍。脂肪生成酶mRNA也显著增加（20至30倍）。这对白色脂肪组织具有特异性，在棕色脂肪组织中未观察到。在肝脏中，高胰岛素血症伴随着葡萄糖激酶（GK）活性和mRNA的三倍增加，以及脂肪生成酶活性和mRNA的三至五倍增加。与脂肪生成活性的变化一致，高胰岛素血症大鼠的白色脂肪组织和肝脏脂肪生成显著增加。数据强烈表明，在大鼠中，胰岛素是导致肝脏和白色脂肪组织中脂质合成增加的驱动力。版权所有（C）1995由W.B.桑德斯公司
Chronic hyperinsulinemia with maintenance of euglycemia was imposed on normal rats for 4 days. In white adipose tissue, hyperinsulinemia resulted in a twofold increase in GLUT4 protein and mRNA and a sixfold to 15-fold increase in fatty acid synthase (FAS) and acetyl coenzyme A (CoA) carboxylase (ACC) activity, respectively. Lipogenic enzyme mRNA was also markedly increased (20 to 30-fold). This was specific for white adipose tissue and was not observed in brown adipose tissue. In the liver, hyperinsulinemia was accompanied by a threefold increase in glucokinase (GK) activity and mRNA and by a threefold to fivefold increase in lipogenic enzyme activities and mRNA. In agreement with the changes in lipogenic activities, lipogenesis was markedly increased in white adipose tissue and liver of hyperinsulinemic rats. The data strongly suggest that in the rat, insulin is a driving force leading to increased lipid synthesis in liver and white adipose tissue. Copyright (C) 1995 by W.B. Saunders Company