Comparative analysis of Bombyx mori nucleopolyhedrovirus responsive genes in fat body and haemocyte of B-mori resistant and susceptible strains
Comparative analysis of Bombyx mori nucleopolyhedrovirus responsive genes in fat body and haemocyte of B-mori resistant and susceptible strains
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DOI：
10.1111/j.1365-2583.2010.00993.x
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发表时间：
2010-06-01
影响因子：
2.6
通讯作者：
Zhang, C-X.
中科院分区：
文献类型：
--
作者：
Bao, Y-Y.;Lv, Z-Y.;Zhang, C-X.
家蚕核型多角体病毒（BmNPV）在B.家蚕幼虫的感染表明，病毒侵入KN和306株的脂肪体和血细胞，这两个株分别对BmNPV感染具有高度抗性和敏感性。然而，在抗性B中病毒增殖显著减慢。森氏菌株。利用抑制性消减杂交技术构建了两个家蚕脂肪体cDNA文库，比较了两个家蚕品系BmNPV应答基因的表达水平。共获得96个差异表达基因。实时荧光定量PCR（qPCR）分析证实，在接种BmNPV后，KN株的脂肪体和血细胞中有8个基因表达显著上调。我们的研究结果表明，这些基因可能有潜在的作用，在B。家蚕抗病毒感染机制
The infection profiles of the Bombyx mori nucleopolyhedrovirus (BmNPV) in B. mori larvae revealed that the virus invaded the fat body and haemocyte of both KN and 306 strains, which are highly resistant and susceptible, respectively, to BmNPV infection. However, viral proliferation was notably slowed in the resistant B. mori strain. Using suppression subtractive hybridization, two fat body cDNA libraries were constructed to compare BmNPV responsive gene expression levels between the two silkworm lines. In total, 96 differentially expressed genes were obtained. Real-time quantitative PCR (qPCR) analysis confirmed that eight genes were significantly up-regulated in the fat body and haemocyte of the KN strain following BmNPV injection. Our results suggest that these genes may have potential roles in B. mori antiviral infection mechanisms.