Beneficial effects of pentoxifylline pretreatment in non-heart-beating donors in rats
Beneficial effects of pentoxifylline pretreatment in non-heart-beating donors in rats
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DOI：
10.1097/00007890-200001150-00017
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发表时间：
2000-01-15
期刊：
影响因子：
6.2
通讯作者：
Astarcioglu, I
中科院分区：
文献类型：
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作者：
Astarcioglu, H;Karademir, S;Astarcioglu, I
背景，戊己茶碱（PTX）预处理受体被证明可以防止大鼠原位肝移植术后缺血再灌注损伤引起的移植肝衰竭。PTX对大鼠肝叶缺血模型中的肝脏常温缺血-再灌注损伤也有保护作用。PTX是否可以使从无心跳供体（NHBDs）获得的肝脏在长达9小时的冷缺血中受益尚不清楚。供体和受体大鼠分别在心脏停搏和移植前1小时腹腔注射PTX（50 mg/kg）。在PTX预处理或未处理（对照）组（每组10只大鼠）中，在心脏停搏后0、30和60分钟分别移植物，并再进行1和9小时的冷缺血。术后连续5 d给予PTX（25 mg/kg/d）治疗，观察大鼠存活率、血清酶水平及肝组织病理学变化。与对照组相比，紫杉醇预处理组的存活率、血清酶水平和再灌注后组织学均显着改善。结论。供体和受体PTX预处理显著提高了从NHBDs获得的肝移植物的存活率。
Background, Pentoxifylline (PTX) pretreatment of recipients was shown to protect against liver graft failure from ischemia-reperfusion injury after orthotopic rat liver transplantation. It has also been shown that PTX protects against normothermic ischemia-reperfusion injury to the liver in lobar ischemia model in the rat. Whether PTX can benefit the liver procured from non-heart-beating donors (NHBDs) with up to 9 hr of cold ischemia is unknown.Methods. Donor and recipient rats were pretreated with intraperitoneal PTX (50 mg/kg) 1 hr before cardiac arrest and transplantation, respectively. Grafts were transplanted 0, 30, and 60 min after cardiac arrest with additional 1 and 9 hr of cold ischemia in both PTX-pretreated or untreated (control) groups (10 rats per group). PTX (25 mg/kg/day) was continuously given to the surviving rats for 5 days postoperatively, Recipient survival rates, serum enzyme levels, and histopathological examination of postreperfusion liver biopsies were all analyzed.Results. The survival rates, serum enzyme levels, and postreperfusion histology were significantly improved in groups pretreated with PTX compared to the controls.Conclusion. Donor and recipient PTX pretreatment significantly improves the viability of the liver grafts procured from NHBDs.