Magnetic Accumulation of SPIONs under Arterial Flow Conditions: Effect of Serum and Red Blood Cells
Magnetic Accumulation of SPIONs under Arterial Flow Conditions: Effect of Serum and Red Blood Cells
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DOI：
10.3390/molecules24142588
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发表时间：
2019-07-02
期刊：
影响因子：
4.6
通讯作者：
Cicha, Iwona
中科院分区：
文献类型：
--
作者：
Hennig, Till L.;Unterweger, Harald;Cicha, Iwona
磁性药物靶向利用外部磁场将超顺磁性氧化铁纳米颗粒（SPION）及其货物靶向到患病的脉管系统区域。在动脉中，流动条件影响磁性颗粒的行为及其积聚的功效。为了估计磁捕获的SPION在更多的生理设置，我们以前建立了一个离体模型的基础上，人类脐带动脉。本研究采用动脉模型，利用2种具有不同理化特性的SPION，分析血液成分对磁靶向疗效的影响。在存在新鲜分离的人血浆或全血的情况下，观察到两种颗粒类型沿着动脉壁的AES测量的铁含量显著增加，与血浆中内源性凝血酶生成引起的凝血激活平行。因此，随后的研究使用血清中的SPION混悬液和红细胞比容50%的洗涤红细胞（RBC）。有趣的是，与细胞培养基悬浮液相反，在RBC存在下实现了循环SPION-3在外部磁体下的磁性积聚。综上所述，我们的数据表明，血液成分的存在会影响，但不会阻止，循环SPION的磁积累。
Magnetic drug targeting utilizes an external magnetic field to target superparamagnetic iron oxide nanoparticles (SPIONs) and their cargo to the diseased vasculature regions. In the arteries, the flow conditions affect the behavior of magnetic particles and the efficacy of their accumulation. In order to estimate the magnetic capture of SPIONs in more physiological-like settings, we previously established an ex vivo model based on human umbilical cord arteries. The artery model was employed in our present studies in order to analyze the effects of the blood components on the efficacy of magnetic targeting, utilizing 2 types of SPIONs with different physicochemical characteristics. In the presence of freshly isolated human plasma or whole blood, a strong increase in iron content measured by AES was observed for both particle types along the artery wall, in parallel with clotting activation due to endogenous thrombin generation in plasma. Subsequent studies therefore utilized SPION suspensions in serum and washed red blood cells (RBCs) at hematocrit 50%. Interestingly, in contrast to cell culture medium suspensions, magnetic accumulation of circulating SPION-3 under the external magnet was achieved in the presence of RBCs. Taken together, our data shows that the presence of blood components affects, but does not prevent, the magnetic accumulation of circulating SPIONs.