Hypoxia induces transcription factor ETS-1 via the activity of hypoxia-inducible factor-1
Hypoxia induces transcription factor ETS-1 via the activity of hypoxia-inducible factor-1
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DOI：
10.1006/bbrc.2001.5927
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发表时间：
2001-11-23
影响因子：
3.1
通讯作者：
Sato, Y
中科院分区：
文献类型：
--
作者：
Oikawa, M;Abe, M;Sato, Y
ETS-1在血管生成和肿瘤侵袭中起重要作用，而缺氧是这些现象的共同特征。我们检测了缺氧是否影响ETS-1的表达。缺氧在人膀胱癌细胞系T24中诱导了ETS-1，启动子分析显示，从人ETS-1启动子中删除-424至-279 bp降低了缺氧介导的诱导能力。该区域包含一个缺氧响应元件样序列，HIF-1在缺氧条件下与之结合。该序列的双链合成寡核苷酸作为诱饵抑制了缺氧介导的诱导。这些结果表明，缺氧通过HIF-1的活性诱导ETS-1。(C) 2001学术出版社。
ETS-1 plays an important role in angiogenesis and cancer invasion, and hypoxia is a common feature in these phenomena. We examined whether hypoxia influenced ETS-1 expression. Hypoxia induced ETS-1 in a human bladder cancer cell line, T24, and promoter analysis revealed that the deletion of -424 to -279 bp from the human ETS-1 promoter decreased the hypoxia-mediated inducibility. This region contained a hypoxia responsive element-like sequence, and HIF-1 bound to it under the hypoxic condition. Double-stranded synthetic oligonucleotides of this sequence as a decoy inhibited the hypoxia-mediated induciblity. These results indicate that hypoxia induces ETS-1 via the activity of HIF-1. (C) 2001 Academic Press.