Dietary dopamine causes ovary activation in queenless Apis mellifera workers
Dietary dopamine causes ovary activation in queenless Apis mellifera workers
复制标题
DOI：
10.1051/apido:2003024
复制
发表时间：
2003-05-01
期刊：
影响因子：
2.4
通讯作者：
Bitondi, MMG
中科院分区：
文献类型：
--
作者：
Dombroski, TCD;Simoes, ZLP;Bitondi, MMG
一组年轻的工蜂被喂食含有多巴胺的食物，并被关在34度和80% RH的小笼子里，在没有蜂王的情况下呆8到13天。在八对笼子里的蜜蜂，每对笼子里有相同数量的工蜂，接受富含花粉的饮食，并补充多巴胺（10杯/克饮食）（DOP组），或不补充（对照组）。在坐月子期间，连续监测饮食的消耗率，之后解剖工人以评估卵巢中的卵泡发育情况。结果显示，与对照组相比，DOP组工人卵巢激活的比例显著高于对照组（P = 0.004）。对照组的蜜蜂存活数量显著高于DOP组（P = 0.01），可能反映了多巴胺的有害作用。两组存活的蜜蜂摄取量相等（P = 0.687），表明卵巢激活不是由于摄取量的差异。研究结果表明，多巴胺在一系列事件中起着调节孤儿蜜蜂工蜂生殖状态变化的作用。
Groups of young honey bee workers were fed a diet containing dopamine while confined in small cages at 34 degreesC and 80% RH in absence of a queen for 8 to 13 days. The bees in eight pairs of cages, each pair containing an equal number of workers, received a pollen-rich diet supplemented with dopamine ( 10 mug/g of diet) (DOP groups), or not supplemented ( controls). The rate of consumption of the diet was monitored continuously during the confinement period, after which the workers were dissected to assess follicle development in the ovaries. The results showed a significantly higher proportion ( P = 0.004) of workers with activated ovaries in the DOP groups than in control groups. The number of bees surviving confinement was significantly higher in the control groups than in the DOP groups ( P = 0.01), possibly reflecting a deleterious effect of dopamine. The surviving bees from both groups consumed equivalent amounts of diet ( P = 0.687), showing that ovary activation was not due to differential diet consumption. The results suggest a role of dopamine in the chain of events mediating changes in the reproductive status of orphan honey bee workers.