Serial multifocal electroretinograms during long-term elevation and reduction of intraocular pressure in non-human primates.
Serial multifocal electroretinograms during long-term elevation and reduction of intraocular pressure in non-human primates.
复制标题
DOI：
10.1007/s10633-010-9231-4
复制
发表时间：
2010-06
影响因子：
1.4
通讯作者：
Hoeve, James N. Ver
中科院分区：
文献类型：
--
作者：
Nork, T. Michael;Kim, Charlene B. Y.;Heatley, Gregg A.;Kaufman, Paul L.;Lucarelli, Mark J.;Levin, Leonard A.;Hoeve, James N. Ver
本研究的目的是评估非人类灵长类动物眼压（IOP）升高与多焦视网膜电图（mfERG）之间的关系。通过激光小梁网破坏（LTD）在 4 只恒河猴和 4 只食蟹猴的一只眼睛中诱发实验性青光眼。为了评估神经节细胞对 mfERG 变化的贡献，每个物种的一只猴子之前都接受了单侧视神经横断（ONT）。在升高 ≥ 44 周后，通过小梁切除术使 2 只未横断的动物的眼压降低。在完整（未横切）的动物中，所有恒河猴（但并非所有食蟹猴）在整个 IOP 升高期间，一级内核 (K1) 的早期 mfERG 波形（N1 和 P1）的振幅均有所增加。物种差异还表现为所有食蟹猴的二级核、第一切片 (K2.1) 的减少，但仅在 1 只恒河猴（带有 ONT 的 1 只）中存在。在 ONT 动物中也观察到了类似的 IOP 对 mfERG 的影响。手术降低眼压导致升高的 K1 振幅恢复至基线水平。然而，食蟹猴中抑制的 K2.1 RMS 并未恢复。这些结果表明，在眼压升高期间，视锥细胞驱动的视网膜功能发生了物种特异性变化。这些与 IOP 相关的影响可能在没有视网膜神经节细胞的情况下发生，并且可能是可逆的。
The purpose of this study was to evaluate the relationship between elevations of intraocular pressure (IOP) and the multifocal electroretinogram (mfERG) in non-human primates. Experimental glaucoma was induced in 4 rhesus and 4 cynomolgus monkeys by laser trabecular meshwork destruction (LTD) in one eye. To evaluate the contribution of ganglion cells to mfERG changes, one monkey of each species had previously underwent unilateral optic nerve transection (ONT). After ≥ 44 weeks of elevation, the IOP was reduced by trabeculectomy in 2 non-transected animals. In the intact (non-transected) animals there was an increase in the amplitude of the early mfERG waveforms (N1 and P1) of the first order kernel (K1) throughout the period of IOP elevation in all of the rhesus, but not all of the cynomolgus monkeys. A species difference was also present as a decrease of the second order kernel, first slice (K2.1) in all of the cynomolgus monkeys but only in 1 of the rhesus monkeys (the 1 with the ONT). Similar IOP effects on the mfERG were seen in the ONT animals. Surgical lowering of IOP resulted in a return of the elevated K1 amplitudes to baseline levels. However, the depressed K2.1 RMS in the cynomolgus monkeys did not recover. These results demonstrate species-specific changes in cone-driven retinal function during periods of elevated IOP. These IOP-related effects can occur in the absence of retinal ganglion cells and may be reversible.
登录
查看更多内容
影响因子：
1.4
作者：
Frishman, Laura J.;Saszik, Shannon;Barnes, George
通讯作者：
Barnes, George
影响因子：
5.1
作者：
Heatley, G;Kiland, J;Nickells, RW
通讯作者：
Nickells, RW
影响因子：
4.4
作者：
Choi, Stacey S.;Zawadzki, Robert J.;Werner, John S.
通讯作者：
Werner, John S.
影响因子：
1.4
作者：
FAZIO, DT;HECKENLIVELY, JR;CHRISTENSEN, RE
通讯作者：
CHRISTENSEN, RE
影响因子：
4.4
作者：
Chauhan, BC;LeVatte, TL;Archibald, ML
通讯作者：
Archibald, ML