Potentiation of Tentacle Ball Formation by a Trypsin-Like Protease and Accompanying Augmented Ingestion in Glutathione-Induced Feeding in Hydra
Potentiation of Tentacle Ball Formation by a Trypsin-Like Protease and Accompanying Augmented Ingestion in Glutathione-Induced Feeding in Hydra
复制标题
胰蛋白酶样蛋白酶增强触手球形成并伴随谷胱甘肽诱导的水螅喂养中的增加摄入
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Y. Matsuoka
中科院分区：
文献类型：
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作者：
K. Hanai;Y. Matsuoka
摘要触手球的形成可能是水螅连续摄食行为的一个组成部分。在0.1 fg/ml至1 μg/ml的浓度范围内，将还原型谷胱甘肽暴露于胰蛋白酶5 min后，可引起这种行为反应。胰蛋白酶和凝血酶增强这种反应比其他蛋白酶检查更有效。胰蛋白酶显着促进消化死亡，固定的虾附着在他们的触角在谷胱甘肽的存在。在一个实际的喂养情况下，胰蛋白酶样蛋白酶，释放活受伤的猎物，可能会加强触手球的形成，因此，摄食的猎物将促进合作与还原型谷胱甘肽。我们发现，单克隆抗体J245和J5的免疫反应性蛋白质在用胰蛋白酶处理的动物中尺寸减小;在没有胰蛋白酶的动物中>300 kDa，而根据胰蛋白酶处理的程度为250 kDa或110 kDa。因此，这种与J245和J5具有免疫反应性的蛋白质可能参与腱球形成的胰蛋白酶依赖性增强和促进摄入。
Abstract Tentacle ball formation may be a component of sequential feeding behavior in Hydra. This behavioral response is elicited by reduced glutathione after exposure to trypsin for 5 min at concentrations ranging from 0.1 fg/ml to 1 μg/ml. Trypsin and thrombin potentiated this response more effectively than the other proteases examined. Trypsin significantly promoted the ingestion of dead, fixed shrimp attached to their tentacles in the presence of glutathione. In an actual feeding situation, a trypsin-like protease, released from living wounded prey, may potentiate tentacle ball formation, and as a result, the ingestion of prey would be promoted in co-operation with reduced glutathione. We found that an immunoreactive protein for the monoclonal antibodies J245 and J5 was reduced in size in animals treated with trypsin; >300 kDa in animals without trypsin vs. 250 kDa or 110 kDa depending on the extent of trypsin treatment. Thus, this protein that is immunoreactive with J245 and J5 is likely to be involved in the trypsin-dependent potentiation of tentacle ball formation and the promotion of ingestion.
丝氨酸蛋白酶刺激仓鼠气管环器官培养物释放粘液糖蛋白。
DOI：
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发表时间：
1986
期刊：
The Journal of laboratory and clinical medicine
影响因子：
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作者：
Niles,RM;Christensen,TG;Breuer,R;Stone,PJ;Snider,GL
通讯作者：
Snider,GL