Pre- and postnatal expression of brain-derived neurotrophic factor mRNA/protein and tyrosine protein kinase receptor B mRNA in the mouse hippocampus
Pre- and postnatal expression of brain-derived neurotrophic factor mRNA/protein and tyrosine protein kinase receptor B mRNA in the mouse hippocampus
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DOI：
10.1016/s0304-3940(01)01905-x
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发表时间：
2001-07-06
影响因子：
2.5
通讯作者：
Beyer, C
中科院分区：
文献类型：
--
作者：
Ivanova, T;Beyer, C
脑源性神经营养因子（BDNF）参与海马发育的控制。本研究采用竞争性逆转录聚合酶链反应（RT-PCR）和酶联免疫分析法，对小鼠海马BDNF mRNA/蛋白及其受体酪氨酸蛋白激酶受体（trk）B mRNA的发育性表达进行了研究。在原代海马培养物中研究BDNF/trkB表达的细胞类型特异性。从胚胎第15天到出生后第二周，BDNF水平逐步增加，而trkB表达在整个发育过程中保持稳定。神经元和星形胶质细胞表达BDNF和trkB。我们的数据表明，发育BDNF的行动主要发生在出生后，并表明海马神经元和星形胶质细胞不仅产生BDNF，但也似乎是BDNF的目标。(C)Elsevier Science爱尔兰有限公司出版。
Brain-derived neurotrophic factor (BDNF) is implicated in the control of hippocampal development. In this study, we have analyzed the developmental expression of BDNF mRNA/protein and its receptor tyrosine protein kinase receptor (trk) B mRNA in the mouse hippocampus by competitive reverse transcription/polymerase chain reaction and enzyme-linked immunoassay. Cell-type specificity of BDNF/trkB expression was studied in primary hippocampal cultures. BDNF levels increased stepwise from embryonic day 15 until the second postnatal week, whereas trkB expression was stable throughout development. Neurons and astrocytes expressed both BDNF and trkB. Our data suggest that developmental BDNF action mainly occurs postnatally and indicates that hippocampal neurons and astroglia not only produce BDNF but also appear to be targets for BDNF. (C) 2001 Published by Elsevier Science Ireland Ltd.