LOSS OF HETEROZYGOSITY OF CHROMOSOME 3P MARKERS IN SMALL-CELL LUNG-CANCER
LOSS OF HETEROZYGOSITY OF CHROMOSOME 3P MARKERS IN SMALL-CELL LUNG-CANCER
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DOI：
10.1038/329451a0
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发表时间：
1987-10-01
期刊：
影响因子：
64.8
通讯作者：
SAKAGUCHI, AY
中科院分区：
文献类型：
--
作者：
NAYLOR, SL;JOHNSON, BE;SAKAGUCHI, AY
特定的染色体缺失有时与肿瘤相关，如视网膜母细胞瘤（染色体13 q14）1和Wilm肿瘤（染色体11 p13）2，这导致了隐性基因可能参与肿瘤发生的假设3。使用多态性DNA标记4 - 9证明了这些区域的等位基因缺失，并分离出视网膜母细胞瘤基因的互补DNA克隆10，从而支持了这一假设。Whang-Peng等人11，12报道了小细胞肺癌（SCLC）中3号染色体（p14-p23）的细胞遗传学缺失。在大多数小细胞肺癌肿瘤中，至少有一个3号染色体同源物受到影响;然而，所观察到的多个染色体变化提出了3号染色体重排而不是缺失的可能性。我们使用了染色体3 p多态性DNA探针，并比较了9例小细胞肺癌患者的肿瘤和体质基因型。我们的数据显示，所有9名患者的肿瘤DNA中染色体3 p标记的等位基因丢失，支持该区域有助于小细胞肺癌肿瘤发生的假设。
Specific chromosomal deletions sometimes associated with tumours such as retinoblastoma (chromosome 13q14)1and Wilm's tumour (chromosome 11p13)2have led to the hypothesis that recessive genes may be involved in tumorigenesis3. This hypothesis is supported by demonstration of allele loss specific for these regions using polymorphic DNA markers4–9and by the isolation of a complementary DNA clone for the retinoblastoma gene10. A cytogenetic deletion in chromosome 3 (p14–p23) was reported in small-cell lung cancer (SCLC) by Whang-Penget al11,12. At least one homologue of chromosome 3 was affected in the majority of SCLC tumours; however, the multiple chromosomal changes seen presented the possibility that chromosome 3 was rearranged, not deleted. We used polymorphic DNA probes for chromosome 3p and compared tumour and constitutional genotypes of nine SCLC patients. Our data show loss of alleles of chromosome 3p markers in tumour DNA of all nine patients supporting the hypothesis that this region contributes to tumorigenesis in SCLC.