Maintenance of presynaptic function by AMPA receptor-mediated excitatory postsynaptic activity in adult brain
Maintenance of presynaptic function by AMPA receptor-mediated excitatory postsynaptic activity in adult brain
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DOI：
10.1073/pnas.0504359103
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发表时间：
2005-12-27
影响因子：
11.1
通讯作者：
Kano, M
中科院分区：
文献类型：
--
作者：
Kakizawa, S;Miyazaki, T;Kano, M
活动依赖性突触修饰发生在发育中和成熟的动物中。然而，为了可靠的信息传输和存储，突触一旦建立，就必须保持稳定。我们研究了长期阻断神经元活动或α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）型谷氨酸受体如何影响成年小鼠小脑的兴奋性攀爬纤维（CF）到浦肯野细胞（PC）突触。两种处理都会导致突触间隙谷氨酸浓度瞬态减少、量子兴奋性突触后电流频率降低、PC 轴树突的 CF 神经支配减少，但 CF 的释放概率没有变化。这些结果表明，在成熟的小脑中，AMPA 受体介导的兴奋性突触后活动维持了 CF 的功能性谷氨酸释放位点及其对 PC 轴树突的神经支配。
Activity-dependent synaptic modification occurs in both developing and mature animals. For reliable information transfer and storage, however, once established, synapses must be maintained stably. We investigated how chronic blockade of neuronal activity or alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors affects excitatory climbing fiber (CF) to Purkinje cell (PC) synapses in adult mouse cerebellum. Both treatments caused reduced glutamate concentration transient at the synaptic cleft, decreased frequency of quantal excitatory postsynaptic current, and diminished CF innervation of PC shaft dendrites but no change in CF's release probability. These results indicate that, in the mature cerebellum, AMPA receptor-mediated excitatory postsynaptic activity maintains CF's functional glutamate-release sites and its innervation of PC shaft dendrites.