Arbuscular mycorrhizal inoculum coupled with organic substrate induces synergistic effects for soil quality changes, and rhizosphere microbiome structure in long-term monocropped cucumber planted soil
Arbuscular mycorrhizal inoculum coupled with organic substrate induces synergistic effects for soil quality changes, and rhizosphere microbiome structure in long-term monocropped cucumber planted soil
复制标题
丛枝菌根接种物与有机基质相结合对长期单作黄瓜种植土壤的土壤质量变化和根际微生物组结构产生协同效应
DOI：
10.1016/j.rhisph.2021.100428
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发表时间：
2021-09-16
期刊：
影响因子：
3.7
通讯作者：
Cai, Zucong
中科院分区：
文献类型：
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作者：
Ali, Ahmad;Ghani, Muhammad Imran;Cai, Zucong
在植物-土壤-微生物的交互作用下，菌根接种与有机基质复合的土壤生物改良剂可表现为加和、协同或拮抗作用。然而，相关的土壤质量变化和微生物群结构的基本机制还不太清楚。为了探讨这一点，连续进行了温室试验（2016-2018），研究了菌根接种物（AMF：Diversispora epigaea L.）和土壤有机基质（GS：蒜薹）进行了单施和配施试验。本研究采用高通量测序技术对连作7年黄瓜根际土壤微生物群落结构进行了分析。两年的处理结果表明，GS + AMF通过提高土壤有机质、养分含量和酶活性，显著改变了土壤质量变化。我们的16 S rRNA扩增子揭示了对α多样性和细菌群落的明显影响。GS改良土壤和GS + AMF接种分别增加了物种丰富度指数（OTUs）、Chao 1和ACE，增加了群落多样性指数（Shannon）。土壤改良剂也有明显的影响，通过改变细菌群落组成在门的水平，这主要是与较高的丰度变形菌，浮游菌，芽单胞菌，放线菌和拟杆菌。此外，相关性分析表明，土壤pH值和酶活性出现的关键微生物属性的主要决定因素。研究结果表明，GS + AMF处理可直接调控黄瓜连作基质的土壤环境属性或诱导土壤微生物组成发生变化。然而，它们的共同接种的影响是关键的土壤微生物群财团的转变。因此，该研究为减轻连作胁迫的不利影响，促进设施栽培微生态的稳定健康发展提供了新的思路。
The integrated soil biological amendments of mycorrhizal inoculation and organic substrate can be additive, synergistic, or antagonistic under plant-soil-microbe interaction. Underlying mechanisms of associated soil quality changes and microbiota structure are, however, less understood. To probe into this, consecutive greenhouse experiments (2016-2018) were conducted to study the effects of mycorrhizal inoculum (AMF: Diversispora epigaea L.) and soil organic substrate (GS: garlic stalk) were used as single and combined applications. In this study, a high-throughput sequencing approach was used to evaluate the rhizosphere microbiome structure of bacteria in the replanted substrate of 7-years of continuous cucumber cropping. From the two years of treatment, we found that co-amended GS + AMF significantly altered the soil quality changes through increasing soil organic matter, soil nutrient contents, and enzyme activities. Our 16 S rRNA amplicon revealed a distinct impact on alpha diversity and bacterial community. The observed species richness index (OTUs, Chao1, and ACE) and community diversity index (Shannon) were increased due to GS-amended soil and GS + AMF inoculation, respectively. Soil amendments also had apparent effects by altering bacterial community compositions at the phylum level, which were mostly associated with the higher abundance of Proteobacteria, Planctomycetes, Gemmatimonadetes, Actinobacteria and Bacteroidetes. Furthermore, correlation analyses revealed that the soil pH and enzyme activities emerged as the major determinants of the key microbial attributes. Our results suggest that GS + AMF treatment can directly regulate the soil environmental attributes or induce compositional shifts of microbes in the continuous cucumber cropping substrates. However, their co-inoculation impact was the shift of the key soil-microbiota consortium. Therefore, the study provides new insight into ways that can reduce the adverse effect of continuous cropping stress and eco-friendly contribute to the stable and healthy micro-ecological development in protected cultivation.