Saccharibacter floricola gen. nov., sp nov., a novel osmophilic acetic acid bacterium isolated from pollen
Saccharibacter floricola gen. nov., sp nov., a novel osmophilic acetic acid bacterium isolated from pollen
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DOI：
10.1099/ijs.0.02911-0
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发表时间：
2004-11-01
影响因子：
2.8
通讯作者：
Fudou, R
中科院分区：
文献类型：
--
作者：
Jojima, Y;Mihara, Y;Fudou, R
从日本花的花粉中分离出三种革兰氏阴性、需氧、杆状细菌菌株，作为木糖醇的生产者;这些菌株进行了多相分类学研究。16 S rRNA基因序列的系统发育分析表明，这三个分离株在α-变形菌门的一组醋酸菌中形成了一个新的簇。这三个菌株的特征如下：（i）它们的主要醌是Q-10;（ii）它们的细胞脂肪酸谱含有大量的2-羟基酸和不饱和直链酸（C-18：1 Ω 7c）;和（iii）它们的DNA G + C含量在51.9- 52.3mol%的范围内，这大约是乙酸细菌属的报道范围的下限。这些菌株从乙醇中生产乙酸的能力可以忽略不计或非常弱，并且嗜酸性生长特性将这些菌株与其他乙酸细菌区分开来。独特的系统发育和表型特征表明，这三个分离物应被归类为一个新的属和种，建议命名为Saccharobacter floricola gen. nov.，模式菌株为S-877（T）（= AJ 13480（T）= JCM 12116（T）= DSM 15669（T））。
Three Gram-negative, aerobic, rod-shaped bacterial strains were isolated, from the pollen of Japanese flowers, as producers of xylitol; these strains were subjected to a polyphasic taxonomic study. Phylogenetic analyses of the 16S rRNA gene sequences demonstrated that these three isolates formed a new cluster within a group of acetic acid bacteria in the alpha-Proteobacteria. The characteristics of the three isolates were as follows: (i) their predominant quinone was Q-10; (ii) their cellular fatty acid profile contained major amounts of 2-hydroxy acids and an unsaturated straight-chain acid (C-18:1 omega7c); and (iii) their DNA G + C contents were in the range 51.9-52.3 mol%, which is around the lower limit of the reported range for the genera of acetic acid bacteria. The negligible or very weak productivity of acetic acid from ethanol and the osmophilic growth properties distinguished these strains from other acetic acid bacteria. The unique phylogenetic and phenotypic characteristics suggest that the three isolates should be classified within a novel genus and species with the proposed name Saccharibacter floricola gen. nov., sp. nov. The type strain is strain S-877(T) ( = AJ 13480(T) = JCM 12116(T) = DSM 15669(T)).