Study of Molecule Variations in Human Xanthelasma Skin Based on Confocal Micro-Raman Spectroscopy
Study of Molecule Variations in Human Xanthelasma Skin Based on Confocal Micro-Raman Spectroscopy
复制标题
基于共焦显微拉曼光谱的人皮肤黄斑瘤分子变异研究
作者：
庄正飞
采用共聚焦微拉曼光谱法对人黄斑瘤皮肤（HXS）和正常皮肤（HNS）进行了区分。结果表明，1,269、1,336、1,448和1,656 cm−1处的密集拉曼峰明显增加。在1,269和1,656 cm−1峰上，HXS中的蛋白质主要呈反平行ß - sheet构象。而HXS光谱中1032、1087、1300、1448 cm−1处属于脂质的波段强度较HNS光谱增强。主要的细胞间脂质是在1087 cm−1和1032 cm−1有少量蛋白质贡献的烷基链。为了定量分析差异，计算比值，HNS和HXS分别为1:1.04±0.04和1:1.11±0.02 （p < 0.01）。数据表明，一些酪氨酸残基在聚集之前与水形成氢键，被聚集体中的强氢键受体捕获。HXS比HNS具有更强的疏水性。主成分分析表明，人黄斑皮肤与相应的拉曼光谱之间存在一一对应的关系。为黄斑病的诊断提供了有益的帮助。扫描37:307-312,2015。©2015 Wiley期刊公司
Confocal micro‐Raman spectroscopy was used to distinguish human xanthelasma skin (HXS) from the human normal skin (HNS). Results showed that intensive Raman peaks at 1,269, 1,336, 1,448, and 1,656 cm−1increased obviously. Both 1,269 and 1,656 cm−1peaks showed that the proteins in HXS were mostly in the anti‐parallel ß sheet conformation. While the intensities of bands at 1,032, 1,087, 1,300, and 1,448 cm−1belonged to lipids were enhanced in HXS spectrum compared to those in HNS spectrum. There were main intercellular lipids alkyl chains with minor proteins contribution at 1,087 cm−1and phenylalanine at 1,032 cm−1. To quantitative analysis of the difference, the ratio ofwas calculated, which was 1:1.04 ± 0.04 and 1:1.11 ± 0.02 for HNS and HXS (p < 0.01), respectively. The data indicated that some tyrosine residues, which form a hydrogen bond with H2O prior to aggregation, were captured by strong hydrogen‐bond acceptors in the aggregate. The decreased ratio ofindicated more hydrophobic in HXS than HNS. Principal component analysis showed a one‐to‐one relationship between human xanthelasma skin and the corresponding Raman spectra. It could be given useful help for the diagnostication of xanthelasma. SCANNING 37:307–312, 2015. © 2015 Wiley Periodicals, Inc.