Proteomic analysis of pathogenesis-related proteins (PRs) induced by compatible and incompatible interactions of pepper mild mottle virus (PMMoV) in Capsicum chinense L3 plants
Proteomic analysis of pathogenesis-related proteins (PRs) induced by compatible and incompatible interactions of pepper mild mottle virus (PMMoV) in Capsicum chinense L3 plants
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DOI：
10.1093/jxb/ern032
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发表时间：
2008-04-01
影响因子：
6.9
通讯作者：
Teresa Serra, Maria
中科院分区：
文献类型：
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作者：
Isabel Elvira, Maria;Molina Galdeano, Myriam;Teresa Serra, Maria
由L-3基因赋予的抗性对大多数辣椒斑驳病毒具有活性，包括西班牙株（PMMoV-S），P-1，P-2致病型，但对辣椒温和斑驳病毒（PMMoV）的某些株（称为P-1，P-2，P-3致病型，如意大利株（PMMoV-I））没有活性。这两种病毒在它们的核苷酸序列水平上几乎相同（98%），并用于攻击携带L-3基因的辣椒PI 159236植物，以进行在该宿主中响应于PMMoV-S或PMMoV-I感染而诱导的PR蛋白的比较蛋白质组学分析。PMMoV-S可诱导C. chinensePI 159236植株叶片上形成坏死的局部病斑，病毒在初侵染部位受到限制。在本文中，C.已经鉴定了属于PR-1、β-1，3-葡聚糖酶（PR-2）、几丁质酶（PR-3）、类胡萝卜素蛋白（PR-5）、过氧化物酶（PR-9）、类萌发蛋白（PR-16）和PRp 27（PR-17）的chinense PR蛋白同种型。这些PR蛋白亚型中的三种在PMMoV-S-激活C. chinense L-3基因介导的抗性：酸性β-1，3-葡聚糖酶同工型（PR-2）（M-r 44.6; pI 5.1）、类胡萝卜素蛋白（PR-5）（M-r 26.8; pI 7.5）和碱性PR-1蛋白同工型（M-r 18; pI 9.4-10.0）。此外，证据提出的差异积累的C。chinense PR蛋白和mRNA在亲和（PMMoV-I）-C. chinense和不亲和（PMMoV-S）-C. chinense相互作用的蛋白质属于所有检测到的PR蛋白。除了酸性几丁质酶（PR-3）（M-r 30.2; pI 5.0），PR蛋白和mRNA的早期和更高的积累与HR诱导植物中检测。此外，PR蛋白和mRNA的积累速率与病毒RNA的最大积累水平无关，因此表明PR蛋白的表达可能反映了植物的生理状态。
Resistance conferred by the L-3 gene is active against most of the tobamoviruses, including the Spanish strain (PMMoV-S), a P-1,P-2 pathotype, but not against certain strains of pepper mild mottle virus (PMMoV), termed P-1,P-2,P-3 pathotype, such as the Italian strain (PMMoV-I). Both viruses are nearly identical at their nucleotide sequence level (98%) and were used to challenge Capsicum chinense PI159236 plants harbouring the L-3 gene in order to carry out a comparative proteomic analysis of PR proteins induced in this host in response to infection by either PMMoV-S or PMMoV-I. PMMoV-S induces a hypersensitive reaction (HR) in C. chinense PI159236 plant leaves with the formation of necrotic local lesions and restriction of the virus at the primary infection sites. In this paper, C. chinense PR protein isoforms belonging to the PR-1, beta-1,3-glucanases (PR-2), chitinases (PR-3), osmotin-like protein (PR-5), peroxidases (PR-9), germin-like protein (PR-16), and PRp27 (PR-17) have been identified. Three of these PR protein isoforms were specifically induced during PMMoV-S-activation of C. chinense L-3 gene-mediated resistance: an acidic beta-1,3-glucanase isoform (PR-2) (M-r 44.6; pI 5.1), an osmotin-like protein (PR-5) (M-r 26.8; pI 7.5), and a basic PR-1 protein isoform (M-r 18; pI 9.4-10.0). In addition, evidence is presented for a differential accumulation of C. chinense PR proteins and mRNAs in the compatible (PMMoV-I)-C. chinense and incompatible (PMMoV-S)-C. chinense interactions for proteins belonging to all PR proteins detected. Except for an acidic chitinase (PR-3) (M-r 30.2; pI 5.0), an earlier and higher accumulation of PR proteins and mRNAs was detected in plants associated with HR induction. Furthermore, the accumulation rates of PR proteins and mRNA did not correlate with maximal accumulation levels of viral RNA, thus indicating that PR protein expression may reflect the physiological status of the plant.