3 CONE MECHANISMS IN THE PRIMATE ELECTRORETINOGRAM - 2 WITH, ONE WITHOUT OFF-CENTER BIPOLAR RESPONSES
3 CONE MECHANISMS IN THE PRIMATE ELECTRORETINOGRAM - 2 WITH, ONE WITHOUT OFF-CENTER BIPOLAR RESPONSES
复制标题
DOI：
10.1016/0042-6989(86)90019-2
复制
发表时间：
1986-01-01
期刊：
影响因子：
1.8
通讯作者：
GOURAS, P
中科院分区：
文献类型：
--
作者：
EVERS, HU;GOURAS, P
我们已经使用ganzfeld刺激，选择性色适应和计算机平均，以确定三个锥机制的猕猴角膜ERG。在强烈的黄色背景上，可以区分出两种锥体机构。其中一种的峰值光谱灵敏度接近460 nm，b波相对缓慢地开始发光，而在偏移处出现负波。这种反应在黄色背景熄灭后会暂时降低。第二个在560 nm附近具有峰值，在开始时具有更快的b波，在偏移时具有正波（d波）。在强蓝色背景下，ERG在400至600 nm之间呈现单变，但在更长的波长（640 nm）下不呈现单变。更亮的蓝色背景将ERG降低到大部分光谱，但对640 nm响应几乎没有影响。添加额外的红色背景大大降低了640 nm的响应，而不会显著影响对光谱其余部分的响应。根据Granit的术语，视锥ERG似乎由短波视锥的类E ERG和至少两种其他视锥机制的类I ERG组成。E和I ERG之间的差异可能分别是由于偏心双极系统的存在或不存在。玻璃体内注射2-氨基-4-膦酰基丁酸（APB）支持这一假设。APB消除了所有三种视锥ERG的b波和S视锥ERG的负性关闭反应，但增加了其他视锥ERG的正性关闭反应。
We have used ganzfeld stimulation, selective chromatic adaptation and computer averaging to identify three cone mechanisms in the corneal ERG of the macaque monkey. On a strong yellow background, two cone mechanisms can be distinguished. One has a peak spectral sensitivity near 460 nm with a relatively slow b-wave to light onset and a negative wave at offset. This response is transiently reduced after a yellow background is extinguished. The second has its peak near 560 nm with a quicker b-wave at onset and a positive wave (d-wave) at offset. On a strong blue background, the ERG appears univariant from 400 to 600 nm but not at longer wavelengths (640 nm). An even brighter blue background reduces the ERG to most of the spectrum but has almost no effect on the 640 nm response. Adding an additional red background greatly reduces the 640 nm response without significantly influencing the responses to the rest of the spectrum. By Granit''s terminology, the cone ERG appears to be composed of an E-like ERG of short wave cones and an I-like ERG of at least two other cone mechanisms. The difference between E and I ERGs may be due to the absence or presence, respectively, of an off-center bipolar system. The intravitreal injection of 2-amino-4-phosphonobutyric acid (APB) supports this hypothesis. APB eliminates the b-wave of all three cone ERGs and the negative off-response of the S cone ERG but increases the positive off-response of the other cone ERGs.