Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films
Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films
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DOI：
10.1002/elan.201000079
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发表时间：
2010-09
期刊：
影响因子：
3
通讯作者：
Zhijie Wang;M. Etienne;G. Kohring;A. Walcarius
中科院分区：
文献类型：
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作者：
Zhijie Wang;M. Etienne;G. Kohring;A. Walcarius
以d -山梨醇脱氢酶（DSDH）为模型酶，研究了聚电解质（PE）添加剂对脱氢酶溶胶-凝胶包封的影响。在玻璃碳电极（GCE）上制备了薄的溶胶-凝胶膜，并对其进行了电化学检测。DSDH包封在纯二氧化硅薄膜中，导致电化学信号无法检测。在生物复合膜中加入带正电荷的PE有助于稳定带负电荷的DSDH，从而使DSDH对底物的响应更快、更高，这可以从d -山梨醇氧化过程中产生的NADH的生物电化学检测得到显著改善。
The influence of polyelectrolyte (PE) additives on the sol-gel encapsulation of dehydrogenases has been evaluated using D-sorbitol dehydrogenase (DSDH) as a model enzyme. Thin sol-gel films were prepared on glassy carbon electrodes (GCE), which were then evaluated with respect to the electrochemical detection of D -sorbitol. DSDH encapsulation in pure silica thin films resulted in undetectable electrochemical signal. Adding positively-charged PE that contributes to stabilize the negatively-charged DSDH in the biocomposite film led to much faster and higher response of DSDH to its substrate, as shown by the significant improvement in bioelectrochemical detection of NADH produced during the oxidation of D-sorbitol.