The labelling index of human and mouse tumours assessed by bromodeoxyuridine staining in vitro and in vivo and flow cytometry.
The labelling index of human and mouse tumours assessed by bromodeoxyuridine staining in vitro and in vivo and flow cytometry.
复制标题
通过体外和体内溴脱氧尿苷染色以及流式细胞术评估人和小鼠肿瘤的标记指数。
DOI：
10.1002/cyto.990060621
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
R. Pfragner
中科院分区：
文献类型：
--
作者：
G. Wilson;N. McNally;E. Dunphy;H. Kärcher;R. Pfragner
溴脱氧尿苷（BrdUrd）掺入和流式细胞术被用来测量人体肿瘤的动力学参数在体外和体内。该技术进行了验证，通过比较标记指数估计的小鼠肿瘤在体内和体外使用BrdUrd和流式细胞术与氚化胸苷（3HdThd）放射自显影。在体内和体外掺入DNA的两种不同的前体获得类似的标记指数。在体外与BrdUrd或3HdThd孵育后，人肿瘤标记指数的测量结果相似。BrdUrd的使用允许显现出不并入BrdUrd或3HdThd的S期细胞群的可视化。用BrdUrd掺入法获得的人类肿瘤标记指数与先前使用放射自显影研究报道的值相似。初步研究表明，静脉注射500 mg BrdUrd可实现显著的人类肿瘤标记。
Bromodeoxyuridine (BrdUrd) incorporation and flow cytometry were used to measure human tumour kinetic parameters in vitro and in vivo. The technique was validated by comparison of labelling index estimates of mouse tumours in vivo and in vitro using BrdUrd and flow cytometry with tritiated thymidine (3HdThd) autoradiography. Similar labelling indices were obtained with both in vivo and in vitro incorporation into DNA of the two different precursors. Measurements of human tumour labelling indices were similar following in vitro incubation with either BrdUrd or 3HdThd. The use of BrdUrd allowed the visualization of a population of S-phase cells that did not appear to incorporate BrdUrd or 3HdThd. The human tumour labelling indices obtained with BrdUrd incorporation were similar to previously reported values using autoradiography studies. Preliminary studies demonstrated that significant human tumour labelling could be achieved with an intravenous injection of 500 mg BrdUrd.