Recent advances in pericentriolar material organization: ordered layers and scaffolding gels.
Recent advances in pericentriolar material organization: ordered layers and scaffolding gels.
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DOI：
10.12688/f1000research.11652.1
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Shackleton S
中科院分区：
文献类型：
--
作者：
Fry AM;Sampson J;Shak C;Shackleton S
中心体是一种不寻常的细胞器，它缺乏周围的膜，这就提出了一个问题，即是什么限制了它的大小和形状。此外，虽然电子显微镜（EM）提供了一个详细的中心粒结构的视图，一直是有限的理解，如何中心体的第二个主要组成部分，的pericentriolar材料（PCM），组织。在这里，我们总结了令人兴奋的超分辨率荧光成像，结构生物学和生化重建，共同揭示了有序层和复杂的凝胶状支架的PCM的存在下，最近的发现。此外，我们还讨论了这是如何导致更好地了解微管成核的过程，PCM大小的改变如何在循环和分化的细胞中进行调节，以及为什么PCM组分的突变会导致特定的人类病理。
The centrosome is an unusual organelle that lacks a surrounding membrane, raising the question of what limits its size and shape. Moreover, while electron microscopy (EM) has provided a detailed view of centriole architecture, there has been limited understanding of how the second major component of centrosomes, the pericentriolar material (PCM), is organized. Here, we summarize exciting recent findings from super-resolution fluorescence imaging, structural biology, and biochemical reconstitution that together reveal the presence of ordered layers and complex gel-like scaffolds in the PCM. Moreover, we discuss how this is leading to a better understanding of the process of microtubule nucleation, how alterations in PCM size are regulated in cycling and differentiated cells, and why mutations in PCM components lead to specific human pathologies.