Patterned paper as a platform for inexpensive, low-volume, portable bioassays
Patterned paper as a platform for inexpensive, low-volume, portable bioassays
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DOI：
10.1002/anie.200603817
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发表时间：
2007-01-01
影响因子：
16.6
通讯作者：
Whitesides, George M.
中科院分区：
文献类型：
--
作者：
Martinez, Andres W.;Phillips, Scott T.;Whitesides, George M.
本文中，我们描述了一种用于使纸图案化以产生明确限定的毫米尺寸通道的简单方法，所述通道包括由疏水性聚合物结合的亲水性纸。我们相信这种类型的图案纸将成为低成本、便携式且技术简单的多重生物测定的基础。我们通过同时检测5 μL尿液中的葡萄糖和蛋白质来证明这种能力。该分析系统体积小，一次性使用，易于使用（和携带），并且不需要外部设备，试剂或电源。我们相信这种系统对于工业化程度较低的国家、在该领域的使用或作为已用于临床环境的更先进技术的廉价替代品是有吸引力的。[1-4]生物液体的分析对于监测人群的健康是必要的，[2]但是这些测量在偏远地区难以实施，例如在工业化程度较低的国家，紧急情况下或家庭卫生保健环境中。[3]常规的实验室仪器提供生物样品的定量测量，但是它们不适合于这些情况，因为它们大、昂贵并且需要训练有素的人员和相当大体积的生物样品。[2]用于生物测定的其他平台提供了更昂贵仪器的替代品，[5-7]但仍然需要使用小体积样品并且足够便宜以广泛使用的平台，特别是在工业化程度较低的国家。我们相信，图案纸可能是最便宜的平台之一，可用于开发检测。我们通过将光致抗蚀剂图案化到层析纸上以形成由疏水线或“壁”分隔的亲水纸的限定区域来制造基于纸的测定装置;这些图案提供生物流体的空间控制，
Herein we describe a simple method for patterning paper to create well-defined, millimeter-sized channels, comprising hydrophilic paper bounded by hydrophobic polymer. We believe that this type of patterned paper will become the basis for low-cost, portable, and technically simple multiplexed bioassays. We demonstrate this capability by the simultaneous detection of glucose and protein in 5 μL of urine. The assay system is small, disposable, easy to use (and carry), and requires no external equipment, reagents, or power sources. We believe this kind of system is attractive for use in lessindustrialized countries, in the field, or as an inexpensive alternative to more-advanced technologies already used in clinical settings.[1–4]The analysis of biological fluids is necessary for monitoring the health of populations,[2] but these measurements are difficult to implement in remote regions such as those found in less-industrialized countries, in emergency situations, or in home health-care settings.[3] Conventional laboratory instruments provide quantitative measurements of biological samples, but they are unsuitable for these situations as they are large, expensive, and require trained personnel and considerable volumes of biological samples.[2] Other platforms for bioassays provide alternatives to more-expensive instruments,[5–7] but the need remains for a platform that uses small volumes of sample and that is sufficiently inexpensive to be widely used, particularly in less-industrialized countries. We believe that patterned paper may be one of the least expensive platforms available for developing assays. We made assay devices based on paper by patterning photoresist onto chromatography paper to form defined areas of hydrophilic paper separated by hydrophobic lines or “walls”; these patterns provide spatial control of biological fluids and