Investigation of Killer Cell Immunoglobulin‐Like Receptors KIR2DL2 and KIR2DL3 Diversity and Identification of Ten Novel KIR2DL3 Alleles in the Chinese Han Population
Investigation of Killer Cell Immunoglobulin‐Like Receptors KIR2DL2 and KIR2DL3 Diversity and Identification of Ten Novel KIR2DL3 Alleles in the Chinese Han Population
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DOI：
10.1111/sji.12277
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发表时间：
2015-04
影响因子：
3.7
通讯作者：
Y. He;S. Tao;Y. Ying;J. He;F. Zhu;H. Lv
中科院分区：
文献类型：
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作者：
Y. He;S. Tao;Y. Ying;J. He;F. Zhu;H. Lv
KIR 2DL 2和KIR 2DL 3是重要的抑制性受体，其识别携带Ser 77和Asn 80的HLA-C等位基因产物的子集。在这项研究中，我们通过基于PCR序列的分型确定了中国汉族人群中KIR 2DL 2和KIR 2DL 3的多样性。在测序的基础上，对166个中国汉族个体的编码区进行了分析，发现了7个新的多态性位点，KIR2DL2*00301，*00101，KIR2DL3*00101，* 00201，*013，*015和10个新的KIR 2DL 3变体（KIR 2DL 3 *00105、00106、00107、00108、019、020、021、022、023和024），其中KIR 2DL 3 *00101是最常见的等位基因。与KIR 2DL 3 *00101序列相比，2DL 3 *00105、2DL 3 *00106、2DL 3 *00107和2DL 3 *00108的所有序列都有一个核苷酸替换（789 T>C、261 C>T、489 G>A和405 G>A），但没有导致氨基酸改变。在2DL 3 *019中的核苷酸位置476、2DL 3 *020中的608 G>A、2DL 3 * 021中的824 T>C和2DL 3 *023中的238 G>A中观察到A>G取代。此外，2DL 3 *022可能来自2DL 3 *00201，在550处具有核苷酸取代G>A。HLA-C1阳性个体多于HLA-C2。本研究获得了中国汉族人群KIR 2DL 2和KIR 2DL 3等位基因多态性数据，并发现了10个新的KIR 2DL 3等位基因。
KIR2DL2 and KIR2DL3 are important inhibitory receptors that recognize a subset of HLA‐C allelic products carrying Ser77 and Asn80. In this study, we have determined KIR2DL2 and KIR2DL3 diversity in the Chinese Han population by a PCR sequence‐based typing. Based on sequencing, the coding regions of 166 Chinese Han individuals, seven new polymorphic sites (238G>A, 405G>A, 476A>G, 550G>A, 608G>A, 789T>C, 947T>C) were found. KIR2DL2*00301, *00101, KIR2DL3*00101,*00201,*013, *015 and ten new KIR2DL3 variants (KIR2DL3*00105, 00106, 00107, 00108, 019, 020, 021, 022, 023 and 024) were identified, of which KIR2DL3*00101 was the most frequent allele. Compared with the sequences of KIR2DL3*00101, all sequences of 2DL3*00105, 2DL3*00106, 2DL3*00107 and 2DL3*00108 had one nucleotide substitution(789T>C, 261C>T, 489G>A and 405G>A),but none resulted in amino acid change. An A>G substitution was observed in nucleotide position 476 in 2DL3*019, 608 G>A in 2DL3*020, 824T>C in 2DL3*021 and 238 G>A in 2DL3*023. In addition, 2DL3*022 probably arose from 2DL3*00201 with a nucleotide substitution G>A at 550. There were more HLA‐C1 positive individuals than HLA‐C2. In conclusion, the data of allelic polymorphism for KIR2DL2 and KIR2DL3 were obtained in the Chinese Han population and ten novel KIR2DL3 alleles were identified.