CAN SENSORY AND MOTOR COLLATERAL SPROUTING BE INDUCED FROM INTACT PERIPHERAL-NERVE BY END-TO-SIDE ANASTOMOSIS
CAN SENSORY AND MOTOR COLLATERAL SPROUTING BE INDUCED FROM INTACT PERIPHERAL-NERVE BY END-TO-SIDE ANASTOMOSIS
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DOI：
10.1016/0266-7681(94)90069-8
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发表时间：
1994-06-01
期刊：
影响因子：
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通讯作者：
KERNS, JM
中科院分区：
文献类型：
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作者：
LUNDBORG, G;ZHAO, Q;KERNS, JM
在大鼠坐骨神经端侧缝合7天前退化或新鲜神经段，研究未损伤坐骨神经完整轴突侧支发芽的可能性。经过14天或35天的恢复期后，对移植的神经段进行按压反射测试，以证明感觉轴突的存在。大多数情况下，使用退化前的神经段而不是新鲜的神经段，反应积极。神经丝染色和组织学检查证实附着神经段存在轴突。在另一系列实验中，将腓近端肌束结扎并切开。7天前退变期后，远端残端端侧与同侧胫束缝合。90天后，对附着部位近端的胫神经进行刺激，可引起原腓肠肌和外胫前肌的明显收缩。这些发现表明，侧枝芽可能发生在完整的轴突上，可能是由附着神经段产生的因素引起的，随后形成功能性的周围连接。
The possibility that collateral sprouting could occur from intact axons in an undamaged sciatic nerve was studied in the rat by suturing either a 7-day predegenerated or a fresh nerve segment in an end-to-side fashion to the sciatic nerve proper. Following a 14- or 35-day recovery period, the pinch reflex test was performed on the transplanted segment to demonstrate the presence of sensory axons, The majority of cases, using a predegenerated nerve segment but not a fresh segment, responded positively. Neurofilament staining and histological examination confirmed the presence of axons in the attached nerve segment. In another series of experiments, the proximal peroneal fascicle was ligated and cut. Following a 7-day predegeneration period the distal stump was sutured end-to-side to the ipsilateral tibial fascicle. After 90 days, stimulation of the tibial nerve proximal to the attached site induced substantial contraction in both the native gastrocnemius muscle and the foreign tibialis anterior muscle. These findings suggest that collateral sprouting may occur from intact axons, perhaps induced by factors emanating from the attached nerve segment, and subsequently make functional peripheral connections.