Micelles assembled with carbocyanine dyes for theranostic near-infrared fluorescent cancer imaging and photothermal therapy.
Micelles assembled with carbocyanine dyes for theranostic near-infrared fluorescent cancer imaging and photothermal therapy.
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DOI：
10.1016/j.biomaterials.2013.08.022
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发表时间：
2013-12
期刊：
影响因子：
14
通讯作者：
Hong Yang;Huajian Mao;Zhihui Wan;Aijun Zhu;M. Guo;Yanli Li;Xinming Li;Jiangling Wan;
中科院分区：
文献类型：
--
作者：
Hong Yang;Huajian Mao;Zhihui Wan;Aijun Zhu;M. Guo;Yanli Li;Xinming Li;Jiangling Wan;
探索一种同时用于癌症成像和治疗的治疗诊断纳米载体是一个新兴的焦点。在此，我们展示了一种用于癌症近红外荧光（NIRF）成像的治疗诊断胶束系统，其具有增强的信噪比和优越的上级光热治疗。由单甲氧基聚乙二醇和烷基胺接枝的聚天冬氨酸组成的共聚物与碳菁染料组装成治疗诊断胶束，该胶束具有小尺寸、高负载量、良好的稳定性、缓释行为和增强的细胞摄取。胶束实现了碳菁染料在肿瘤上的优选生物分布和长期保留，这通过在长达8天的长期成像期间在多血管和少血管肿瘤处产生稳定的NIRF信号而导致增强的NIRF成像，同时在正常组织处产生可忽略的噪声。碳菁染料（Cypate）的光稳定性对于增强SNR的长期癌症成像发挥着重要作用。此外，胶束在光照射时通过亚细胞器的不稳定性对癌细胞表现出严重的光热损伤，导致上级光热肿瘤消退。胶束作为一个强大的治疗诊断纳米载体，用于同时进行高对比度和上级光热治疗的癌症成像。
It is an emerging focus to explore a theranostic nanocarrier for simultaneous cancer imaging and therapy. Herein, we demonstrate a theranostic micelle system for cancer near infrared fluorescent (NIRF) imaging with enhanced signal to noise ratio and superior photothermal therapy. The copolymers consisting of monomethoxy poly(ethylene glycol) and alkylamine-grafted poly(l-aspartic acid) are assembled with carbocyanine dyes into theranostic micelles, which exhibit small size, high loading capacity, good stability, sustained release behavior, and enhanced cellular uptake. The micelles achieve the preferable biodistribution and long-term retention of carbocyanine dyes at tumor, which result in enhanced NIRF imaging by generating stable retention of NIRF signals at both hypervascular and hypovascular tumors during a long-term imaging period of up to 8 day, accompanying with negligible noise at normal tissues. The photostability of carbocyanine dye (Cypate) plays an important role for long-term cancer imaging with enhanced SNR. Moreover, the micelles exhibit severe photothermal damage on cancer cells via the destabilization of subcellular organelles upon photoirradiation, causing superior photothermal tumor regress. The micelles act as a powerful theranostic nanocarrier for simultaneous cancer imaging with high contrast and superior photothermal therapy.