PATHOGENESIS OF PROVIDENCIA-ALCALIFACIENS-INDUCED DIARRHEA
PATHOGENESIS OF PROVIDENCIA-ALCALIFACIENS-INDUCED DIARRHEA
复制标题
DOI：
10.1128/iai.60.12.5017-5024.1992
复制
发表时间：
1992-12-01
影响因子：
3.1
通讯作者：
MATHAN, MM
中科院分区：
文献类型：
--
作者：
ALBERT, MJ;ALAM, K;MATHAN, MM
产碱普罗威登斯菌是肠杆菌科的成员。有报道称产碱假单胞菌可引起腹泻，但其引起腹泻的机制尚不清楚。我们研究了从一名儿童和两名成人腹泻分离的产碱假单胞菌的肠道致病性。这三个分离株没有表现出任何特征性的粘附培养的HEp-2细胞单层，他们没有产生肠毒素，细胞毒素，或角膜结膜炎在Sereny测试。两个菌株侵入培养的HEp-2细胞单层，产生局部细菌簇和肌动蛋白凝聚。肌动蛋白凝聚的模式是不同的肠致病性大肠杆菌产生的，但类似于志贺氏菌产生的。第三个分离物的侵袭力和肌动蛋白凝聚力较差。接种所有三种分离株的成年家兔小肠袢的组织学显示细菌附着、渗透和表面上皮上的微溃疡形成以及固有层的充血、水肿和多形细胞浸润。所有分离株在具有可移除肠结的兔中产生腹泻，并且这些兔中的一些发生后肢麻痹。发生腹泻的具有可移除肠结的兔的肠组织学显示与已进行回肠袢测定的成年兔相似的变化。肠组织的透射电子显微镜也证实了分离株的组织渗透。神经组织学显示，2只兔后肢瘫痪周围周围神经鞘局灶性单核细胞浸润。结论：产碱假单胞菌的某些菌株是肠致病性的，它们通过侵入肠粘膜上皮引起腹泻。然而，在该动物模型中观察到的后肢瘫痪与人类疾病的相关性尚不清楚。
Providencia alcalifaciens is a member of the family Enterobacteriaceae. There are reports that P. alcalifaciens can cause diarrhea, but the mechanism(s) by which it causes diarrhea is not known. We studied P. alcalifaciens isolated from a child and two adults with diarrhea for enteropathogenicity. The three isolates did not exhibit any characteristic adherence to cultured HEp-2 cell monolayers, and they did not produce enterotoxins, cytotoxins, or keratoconjunctivitis in the Sereny test. Two isolates invaded cultured HEp-2 cell monolayers, producing localized bacterial clusters and actin condensation. The pattern of actin condensation was different from that produced by enteropathogenic Escherichia coli but similar to that produced by Shigella flexneri. Invasion and actin condensation were poor for the third isolate. Histology of adult rabbit small intestinal loops inoculated with all three isolates revealed bacterial attachment to, penetration of, and microulcer formation on the surface epithelium and hyperemia, edema, and polymorphonuclear cell infiltration of lamina propria. All the isolates produced diarrhea in rabbits with removable intestinal ties, and some of these rabbits developed hindlimb paralysis. Intestinal histology of the rabbits with removable intestinal ties which developed diarrhea showed changes similar to that in adult rabbits on which ileal loop assays had been performed. Transmission electron microscopy of intestinal tissues also confirmed tissue penetration by the isolates. Nerve tissue histology of two rabbits that developed hindlimb paralysis showed focal mononuclear cell infiltration around peripheral nerve sheaths. It is concluded that some strains of P. alcalifaciens are enteropathogenic and that they cause diarrhea by invading the intestinal mucosal epithelium. However, the relevance to human disease of the hindlimb paralysis observed in this animal model is not clear.