Study of the genetic stability of measles virus CAM-70 vaccine strain after serial passages in chicken embryo fibroblasts primary cultures
Study of the genetic stability of measles virus CAM-70 vaccine strain after serial passages in chicken embryo fibroblasts primary cultures
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DOI：
10.1007/s11262-007-0173-1
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发表时间：
2008-02-01
期刊：
影响因子：
1.6
通讯作者：
Galler, Ricardo
中科院分区：
文献类型：
--
作者：
Borges, Maria Beatriz;Caride, Elena;Galler, Ricardo
为了评价CAM-70麻疹疫苗株的遗传稳定性，我们在生产条件下，在鸡胚成纤维细胞原代培养物（CEF）中对种子批病毒FMS-7进行了10次连续传代。测定种子批病毒、疫苗小瓶（第3代）和第10代病毒的核苷酸序列，并相互比较，并与其他来源的序列进行比较。CAM-70疫苗的全基因组分析仍然认为它是所有疫苗株中最具差异性的。来自三个CAM-70传代水平的病毒基因组的核苷酸序列分析证明它们是相同的。这项研究表明，麻疹CAM-70疫苗病毒高度适应其培养条件，并且其遗传稳定性在一定程度上有助于疫苗的安全性。
To evaluate the genetic stability of the CAM-70 measles vaccine strain we have performed 10 serial passages of the seed lot virus FMS-7 in chicken embryo fibroblasts primary cultures (CEF) under production conditions. The nucleotide sequences of the seed lot virus, the virus from a vaccine vial (third passage) and from the 10th passage were determined and compared with each other and with sequences from other sources. The full genome analysis of the CAM-70 vaccine still considers it as the most divergent among all vaccine strains. The nucleotide sequence analyses of viral genomes from the three CAM-70 passage levels have demonstrated that they are identical. This study shows that the measles CAM-70 vaccine virus is highly adapted to its cultivation conditions and that its genetic stability contributes, in part, to the safety profile of the vaccine.