Californium-252 plasma desorption time of flight mass spectroscopy of proteins.
Californium-252 plasma desorption time of flight mass spectroscopy of proteins.
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Californium-252 蛋白质的等离子体解吸飞行时间质谱。
DOI：
10.1002/bms.1200110509
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Peter Roepstorff
中科院分区：
文献类型：
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作者：
B. Sundqvist;I. Kamensky;P. Håkansson;J. Kjellberg;M. Salehpour;S. Widdiyasekera;Jan Fohlman;P. Peterson;Peter Roepstorff
快重离子，即来自252 Cf源的裂变碎片，已被用于从样品表面解吸和吸附肽和蛋白质。质量的解吸离子已被确定的飞行时间技术。所研究的分子质量区间为1000- 14000 u.已经观察到了比先前报道的质量更高的准分子离子。结果包括胰岛素原、细胞色素C、核糖核酸酶和两种磷脂酶的准分子离子的检测。描述了用这种电离方法得到的蛋白质质谱的一般特征。重点讨论了亚稳离子衰变、中性成分、多电荷离子、同位素展宽和团簇离子的形成。此外，可以得到一个直接的飞行时间质谱仪的精度进行了讨论。该技术的未来应用进行了概述。
Fast heavy ions, i.e. fission fragments from a 252Cf-source, have been used to desorb and ionize peptides and proteins from a sample surface. Masses of the desorbed ions have been determined by the time-of-flight technique. The mass interval of the molecules studied is 1000-14 000 u. Quasi-molecular ions of higher masses than earlier reported have been observed. The results include the detection of quasi-molecular ions of proinsulins, cytochrome-C, ribonuclease and two phospholipases. The general features of mass spectra of proteins using this ionization method are described. Emphasis is put on the discussion of metastable ion decay, neutral components, multiply charged ions, isotopic broadening, and cluster ion formation. Also the precision which can be obtained with a straight time-of-flight mass spectrometer will be discussed. Future applications of the technique are outlined.