Isolation and identification of potash feldspar-solubilizing bacteria and their potassium-releasing activities
Isolation and identification of potash feldspar-solubilizing bacteria and their potassium-releasing activities
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发表时间：
2012
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通讯作者：
Peng Qing-jing
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作者：
Peng Qing-jing
目的筛选高活性解钾长石细菌，并对其进行初步鉴定和释钾活性测定。方法采集湖南省桂东县钾矿区土壤样品，以钾长石为唯一钾源，采用富集培养法分离解钾长石细菌，通过释钾试验筛选高效解钾细菌，同时，对分离菌株进行初步鉴定，并测定其释钾活性。结果从土壤样品中分离到11株解钾细菌，其中4株解钾细菌的产钾能力较强，而11株解钾细菌的产钾能力较弱结果表明，JKC 1、JKC 2、JKC 5和JKC 7具有较强的解钾能力，其解钾率分别为11.00%、11.50%、12.70%和11.70%，经鉴定为巨大芽孢杆菌（Bacillusmegaterium）、阿氏芽孢杆菌（Bacillusaryabhattai）、结论JKC 1、JKC 2、JKC 5和JKC 7是高效的钾长石溶解菌，可作为钾长石微生物漂白机理研究的候选菌株。
Objective To screen highly-active potash feldspar-solubilizing bacteria,identify the isolates preliminarily and determine their potassium-releasing activities.Methods The soil samples were collected from the potassium mine region of Guidong County,Hunan Province.Potash feldspar-solubilizing bacteria were isolated by enrichment culture using potash feldspar as a sole potassium resource,and efficiently potassium-solubilizing bacteria were screened by potassium-releasing experiments.Meanwhile,potassium-releasing rate of potash feldspar-solubilizing bacteria was determined by ICP-OES.The isolates were identified by bacterial morphology,physiology and biochemical characteristics,and phylogenetic relationship based on 16S rRNA gene sequences.Results 11 strains of potash feldspar-solubilizing bacteria were isolated from the soil samples,4 of which(JKC1,JKC2,JKC5 and JKC7) had high ability of releasing potassium,and the rates were 11.00%,11.50%,12.70% and 11.70% respectively.JKC1,JKC2,JKC5 and JKC7 were identified as Bacillus megaterium,Bacillus aryabhattai,Azotobacter chroococcum and Microbacterium trichothecenolyticum.Conclusion JKC1,JKC2,JKC5 and JKC7 are high-efficient potash feldspar-solubizing bacteria,and could be used as candidate stains to investigate the microbial bleaching mechanism of potash feldspar.