Hydroxyhydroquinone, a by-product of coffee bean roasting, increases intracellular Ca2+ concentration in rat thymic lymphocytes
Hydroxyhydroquinone, a by-product of coffee bean roasting, increases intracellular Ca2+ concentration in rat thymic lymphocytes
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DOI：
10.1016/j.fct.2017.01.025
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发表时间：
2017-04-01
影响因子：
4.3
通讯作者：
Oyama, Yasuo
中科院分区：
文献类型：
--
作者：
Kamae, Risa;Nojima, Shoko;Oyama, Yasuo
羟基对苯二酚(HHQ)是在咖啡豆烘焙过程中产生的。一杯咖啡含有0.1，1.7毫克的HHQ。由于HHQ是导致白血病的苯的代谢物，因此研究了HHQ对哺乳动物DNA的作用。目前，关于HHQ的细胞活动的信息有限。我们用荧光探针的流式细胞仪技术研究了亚致死剂量的HHQ对大鼠胸腺淋巴细胞内钙离子浓度的影响。10 mM或更高浓度的HHQ通过增加二价阳离子的膜通透性，使细胞内钙离子水平显著升高，并通过激活钙依赖的K+通道而导致超极化。HHQ引起的细胞内钙离子浓度和膜电位的改变可能影响淋巴细胞的细胞功能。HHQ；为了避免HHQ可能产生的不利影响，减少咖啡可能是更好的选择。(C)2017爱思唯尔有限公司。保留所有权利。
Hydroxyhydroquinone (HHQ) is generated during coffee bean roasting. A cup of coffee contains 0.1 1.7 mg of HHQ. The actions of HHQ on mammalian DNA were examined because HHQis a Metabolite of benzene, which causes leukemia. Currently, information on the cellular actions of HHQ is limited. We examined the effects of sublethal levels of HHQ on the concentration of intracellular Ca2+ in rat thymic lymphocytes by using a flow cytometric technique with fluorescent probes. HHQ at 10 mu M or more significantly elevated intracellular Ca2+ levels by increasing the membrane permeability of divalent cations, resulting in hyperpolarization via the activation of Ca2+-dependent K+ channels. HHQ-induced changes in the intracellular Ca2+ concentration and membrane potential may affect the cell functions of lymphocytes. HHQ; reduced coffee may be preferable in order to avoid the possible adverse effects of HHQ. (C) 2017 Elsevier Ltd. All rights reserved.