IN-VITRO EVALUATION OF CYTOTOXICITY OF DIEPOXY COMPOUNDS USED FOR BIOMATERIAL MODIFICATION
IN-VITRO EVALUATION OF CYTOTOXICITY OF DIEPOXY COMPOUNDS USED FOR BIOMATERIAL MODIFICATION
复制标题
DOI：
10.1002/jbm.820290707
复制
发表时间：
1995-07-01
期刊：
影响因子：
--
通讯作者：
IKADA, Y
中科院分区：
文献类型：
--
作者：
NISHI, C;NAKAJIMA, N;IKADA, Y
采用细胞培养方法研究了用于生物材料交联的各种二环氧化合物的毒性，并与体内方法进行了比较。细胞摄取的中性红用于计数与二环氧化合物接触后仍然存活的细胞数量，因为该方法在细胞计数方面比本工作中研究的其他四种方法更灵敏。与其他方法相比，细胞吸收的中性红量很大程度上取决于细胞的活性；当细胞处于低活性状态时，即使它们还活着，也只会吸收少量的中性红。中性红法评价的二环氧化合物的体外毒性与体内Draize试验结果具有良好的相关性。 L929细胞系的体外细胞毒性与正常兔角膜表皮细胞原代培养细胞的细胞毒性密切相关。双环氧化合物的毒性随着其链越长而越低，这可能是因为化学反应性较低。本研究中研究的所有二环氧化合物均表现出比甲醛、戊二醛和水溶性碳二亚胺更低的细胞毒性。 (C) 1995 约翰威利父子公司
The toxicity of various diepoxy compounds used for biomaterials crosslinking was investigated with a cell culture method and compared with an in vivo method. The neutral red uptake by cells was used to count the number of cells still alive after contact with the diepoxy compounds, because this method was more sensitive in cell counting than the other four methods studied in this work. The amount of neutral red taken up by cells depended strongly on the activity of cells in comparison with other methods; only small amounts of neutral red were taken up when cells were in a low activity state even if they were still alive. The in vitro toxicity of diepoxy compounds evaluated by the neutral red method revealed a good correlation with that found by the in vivo Draize test. The in vitro cytotoxicity to a cell line of L929 was closely related to that of primary culture cells of the normal rabbit cornea epidermal cell. The toxicity of diepoxy compounds was lower as their chain was longer, probably because of the lower chemical reactivity. All the diepoxy compounds investigated in this study exhibited lower cytotoxicity than formaldehyde, glutaraldehyde, and a water-soluble carbodiimide. (C) 1995 John Wiley and Sons, Inc.