Relationship between NFKB1-94 insertion/deletion ATTG polymorphism and susceptibility of cervical squamous cell carcinoma risk
Relationship between NFKB1-94 insertion/deletion ATTG polymorphism and susceptibility of cervical squamous cell carcinoma risk
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NFKB1-94插入/缺失ATTG多态性与宫颈鳞癌易感性的关系
DOI：
10.1093/annonc/mdp507
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发表时间：
2010-03-01
影响因子：
50.5
通讯作者：
Zhang, L.
中科院分区：
文献类型：
--
作者：
Zhou, B.;Qie, M.;Zhang, L.
背景资料：已经观察到核因子-κ B蛋白（核p50，由NF κ B 1编码）在高度鳞状上皮内病变和浸润性癌中非常高的表达。为探讨NFKB 1 294插入/缺失ATTG基因多态性（rs 28362491）与宫颈鳞状细胞癌（CSCC）的关系，采用聚合酶链反应-聚丙烯酰胺凝胶电泳法对233例CSCC患者和365例健康对照妇女进行NFKB 1 294插入/缺失ATTG基因多态性分析。基因分型方法通过DNA测序分析得到证实。CSCC患者ATTG（2）/ATTG（2）基因型和ATTG（2）等位基因频率显著高于对照组，提示NFKB 1基因启动子294插入/缺失ATTG多态性与CSCC相关[P = 0.001，OR = 2.560，95%可信区间（CI）1.459-4.492和P = 0.001，OR = 1.493，95% CI 1.168-1.908]。分层分析的结果显示，这种多态性与年轻年龄（
Background: A very high expression of nuclear factor-kappa B protein (nuclear p50, encoded by NFKB1) in high-grade squamous intraepithelial lesion and invasive cancers has been observed. The aim of this study was to determine whether the functional NFKB1 294 insertion/deletion ATTG polymorphism (rs28362491) is associated with cervical squamous cell carcinoma (CSCC).Materials and methods: PCR-polyacrylamide gel electrophoresis method was used to genotype the NFKB1 294 insertion/deletion ATTG polymorphism in 233 women with CSCC and 365 ethnicity-matched healthy control women. The genotyping method was confirmed by the DNA sequencing analysis.Results: The frequency of ATTG(2)/ATTG(2) genotype and ATTG(2) allele in the CSCC patients was significantly higher than that of controls, indicating that the 294 insertion/deletion ATTG polymorphism in NFKB1 promoter was associated with CSCC [P = 0.001, odds ratio (OR) = 2.560, 95% confidence interval (CI) 1.459-4.492 and P = 0.001, OR = 1.493, 95% CI 1.168-1.908, respectively]. Results of stratified analyses revealed that this polymorphism is associated with younger age (