The response of annulus fibrosus cell to fibronectin-coated nanofibrous polyurethane-anionic dihydroxyoligomer scaffolds
The response of annulus fibrosus cell to fibronectin-coated nanofibrous polyurethane-anionic dihydroxyoligomer scaffolds
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DOI：
10.1016/j.biomaterials.2010.09.010
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发表时间：
2011-01-01
期刊：
影响因子：
14
通讯作者：
Kandel, Rita A.
中科院分区：
文献类型：
--
作者：
Attia, Menat;Santerre, J. Paul;Kandel, Rita A.
纤维环（AF）是椎间盘的一个组成部分，由于其复杂的定向板层结构，其组织工程已被证明是具有挑战性的。之前的研究表明，含有阴离子二羟基低聚物（ADO）的聚氨酯（PU）支架可能适合用于这一应用。目前的研究探讨了基质蛋白涂层（I型胶原蛋白I型胶原蛋白和纤维连接蛋白或玻璃体连接蛋白）是否会促进细胞和胶原的定向更接近天然AF的细胞附着发生在预先包被Fn的支架上，Fn包被支架上的细胞与支架纤维平行排列，这一过程涉及整合素特异性阻断抗体确定的α 5 β 1整合素，这反过来减少AF细胞的扩散和排列，细胞形状由肌动蛋白细胞骨架调节，因为细胞在细胞松弛素D存在下生长，细胞在形成的Fn包被PU支架上不扩散与生长在其他蛋白包被支架和未包被对照上的细胞相比，纤维性Fn合成了更多的胶原蛋白，并显示出分泌的I型胶原蛋白的线性排列。因此，PU-ADO支架的Fn包被似乎促进了正确定向的AF细胞和胶原蛋白，这将促进AF组织的发育，更接近于模仿天然组织(C) 2010
Tissue engineering of the annulus fibrosus(AF) a component of the intervertebral disc has proven to be challenging due to its complex oriented lamellar structure Previously it was demonstrated that polyurethane (PU) scaffolds containing an anionic dihydroxy oligomers (ADO) may be suitable to use in this application The current study examines whether matrix protein(s) coatings (collagen type I collagen type I and fibronectin fibronectin or vitronectin) would promote cell and collagen orientation that more closely mimics native AF The greatest cell attachment occurred when scaffolds were pre-coated with Fn Cells on Fn-coated scaffolds were aligned parallel to scaffold fibers a process that Involved alpha 5 beta 1 integrin as determined by integrin-specific blocking antibodies which in turn reduced AF cell spreading and alignment Cell shape was regulated by the actin cytoskeleton as cells grown in the presence of cytochalasin D did not spread Cells on Fn-coated PU scaffolds formed fibrillar Fn synthesized significantly more collagen and showed linear alignment of the secreted type I collagen when compared to cells grown on the other protein-coated scaffolds and the non-coated control Thus Fn-coating of PU-ADO scaffolds appears to promote properly oriented AF cells and collagen which should facilitate developing AF tissue that more closely mimics the native tissue (C) 2010 Published by Elsevier Ltd