TRANSGENIC PRODUCTION OF A VARIANT OF HUMAN TISSUE-TYPE PLASMINOGEN-ACTIVATOR IN GOAT MILK - GENERATION OF TRANSGENIC GOATS AND ANALYSIS OF EXPRESSION
TRANSGENIC PRODUCTION OF A VARIANT OF HUMAN TISSUE-TYPE PLASMINOGEN-ACTIVATOR IN GOAT MILK - GENERATION OF TRANSGENIC GOATS AND ANALYSIS OF EXPRESSION
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DOI：
10.1038/nbt0991-835
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发表时间：
1991-09-01
期刊：
影响因子：
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通讯作者：
GORDON, K
中科院分区：
文献类型：
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作者：
EBERT, KM;SELGRATH, JP;GORDON, K
我们报告了第一次成功生产的转基因山羊，在他们的牛奶中表达一种异种蛋白。利用含有小鼠乳清酸启动子（WAP）的表达载体，研究了在转基因奶山羊中产生人tPA糖基化变体（LAtPA -长效组织型纤溶酶原激活物）的情况。WAP与人tPA的修饰版本cDNA有操作连接。从29只出生的山羊中鉴定出两只转基因山羊。第一只动物，一只雌性，被交配并允许怀孕至足月。在分娩时获得的牛奶被证明含有酶活性LAtPA，浓度为3 μ g/ml。
We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA - longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic goats were identified from 29 animals born. The first animal, a female, was mated and allowed to carry the pregnancy to term. Milk was obtained upon parturition and was shown to contain enzymatically active LAtPA at a concentration of 3-mu-g/ml.