Transcriptional and physiological study of the response of Burma mangrove (Bruguiera gymnorhiza) to salt and osmotic stress
Transcriptional and physiological study of the response of Burma mangrove (Bruguiera gymnorhiza) to salt and osmotic stress
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DOI：
10.1007/s11103-008-9356-y
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发表时间：
2008-09-01
影响因子：
5.1
通讯作者：
Tada, Yuichi
中科院分区：
文献类型：
--
作者：
Miyama, Masashi;Tada, Yuichi
我们研究了转录响应缅甸红树林（木榄）高盐（盐胁迫; 500 mM NaCl）和高渗胁迫（渗透胁迫; 1 M山梨醇）的微阵列分析。方差分析（P < 0.05）和微阵列显著性分析（SAM; FDR < 5%）显示，11,997个基因中有865个在盐和渗透胁迫下表现出显著差异表达。散点图分析表明，盐胁迫后6 h各基因表达水平发生变化，24 h后恢复，而渗透胁迫后6 h的变化在24 h出现分化。对865个基因进行系统聚类分析，结果表明，盐胁迫下的表达谱与渗透胁迫下的表达谱存在明显差异。比较胁迫条件下差异表达基因的基因本体（GO）分类发现，缅甸红树林对盐胁迫的适应伴随着“细胞通讯”、“信号转导”、“脂质代谢过程”、“光合作用”、“多细胞生物发育”和“运输，以及通过下调分解代谢过程的基因。“缅甸红树林保持其叶水势，并从盐胁迫下暂时下降的光合速率中恢复过来，但在渗透胁迫下却没有。这些结果表明，盐和渗透胁迫的反应之间的根本区别。离子和糖含量分析表明，缅甸红树林的耐盐性可能归因于其积累高浓度的Na（+）和Cl（-）的能力，即使在非胁迫条件下，吸收额外的Na（+）和Cl（-）作为渗透剂使用，并在盐胁迫下维持K（+）的动态平衡。
We investigated the transcriptional response of Burma mangrove (Bruguiera gymnorhiza) to high salinity (salt stress; 500 mM NaCl) and hyperosmotic stress (osmotic stress; 1 M sorbitol) by microarray analysis. ANOVA (P < 0.05) and significant analysis of microarray (SAM; FDR < 5%) revealed that 865 of 11,997 genes showed significant differential expression under salt and osmotic stress. Scatter plot analysis revealed that the expression level of genes changed at 6 h after salt stress treatment, but recovered at 24 h, while the change at 6 h after osmotic stress treatment diverged at 24 h. Hierarchical clustering of the 865 genes showed that expression profiles under salt stress were distinctly different from those under osmotic stress. Comparison of gene ontology (GO) categories of differentially expressed genes under the stress conditions revealed that the adaptation of Burma mangrove to salt stress was accompanied by the up-regulation of genes categorized for "cell communication," "signal transduction," "lipid metabolic process," "photosynthesis," "multicellular organismal development," and "transport," and by down-regulation of genes categorized for "catabolic process." Burma mangrove maintained its leaf water potential and recovered from its photosynthesis rate that declined temporarily under salt stress, but not under osmotic stress. These results demonstrated a fundamental difference between the response to salt and osmotic stress. Ion and sugar content analysis suggested that salt tolerance of Burma mangrove might be attributed to their ability to accumulate high concentrations of Na(+) and Cl(-), even under non-stressed conditions; to uptake additional Na(+) and Cl(-) for use as osmolytes; and to maintain K(+) homeostasis under salt stress.