Drosophila fasciclin I is a novel homophilic adhesion molecule that along with fasciclin III can mediate cell sorting.
Drosophila fasciclin I is a novel homophilic adhesion molecule that along with fasciclin III can mediate cell sorting.
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DOI：
10.1083/jcb.110.5.1825
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发表时间：
1990-05
影响因子：
7.8
通讯作者：
Goodman, C S
中科院分区：
文献类型：
--
作者：
Elkins, T;Hortsch, M;Bieber, A J;Snow, P M;Goodman, C S
Fasciclin I是一种膜相关糖蛋白，在昆虫神经元发育过程中局部表达在束状轴突的亚群上；它表达在相对的细胞表面，表明它在特定的细胞黏附中发挥作用。在这篇文章中，我们证明了果蝇Fasciclin I是一种新的同嗜性细胞黏附分子。当非粘附性果蝇S2细胞被转染Fasciclin I基因时，它们形成的聚集体被抗Fasciclin I的抗血清所阻断。当表达Fasciclin I的细胞与表达Fasciclin III的细胞混合时，这种混合物被分拣成对于表达Fasciclin I或Fasciclin III的细胞来说是均一的聚集体。这两种新型黏附分子在体外介导细胞分选的能力表明，它们可能在神经元发育过程中发挥类似的作用。
Fasciclin I is a membrane-associated glycoprotein that is regionally expressed on a subset of fasciculating axons during neuronal development in insects; it is expressed on apposing cell surfaces, suggesting a role in specific cell adhesion. In this paper we show that Drosophila fasciclin I is a novel homophilic cell adhesion molecule. When the nonadhesive Drosophila S2 cells are transfected with the fasciclin I cDNA, they form aggregates that are blocked by antisera against fasciclin I. When cells expressing fasciclin I are mixed with cells expressing fasciclin III, another Drosophila homophilic adhesion molecule, the mixture sorts into aggregates homogeneous for either fasciclin I- or fasciclin III-expressing cells. The ability of these two novel adhesion molecules to mediate cell sorting in vitro suggests that they might play a similar role during neuronal development.