Transcriptome sequencing reveals the characteristics of spermatogenesis and testis development in Amphioctopus fangsiao
Transcriptome sequencing reveals the characteristics of spermatogenesis and testis development in Amphioctopus fangsiao
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DOI：
10.1016/j.aqrep.2024.101957
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发表时间：
2024-02-06
影响因子：
3.7
通讯作者：
Yang，Jianmin
中科院分区：
文献类型：
--
作者：
Jiang，Yu;Sun，Xiaojie;Yang，Jianmin
精子发生是雄性动物睾丸产生精子的过程，方孝端章鱼（Amphipoctopus fangsiao）是我国北方沿海重要的经济鱼类，但对其精子发生的研究较少。在这项研究中，我们进行了睾丸和其他体细胞组织的转录组分析。选择精巢发育的成熟期，观察精子的出现。共鉴定出15，773个差异表达基因，其中上调基因8，922个，下调基因6，851个。通过GO和KEGG富集分析探讨精子发生相关基因的功能，并通过构建蛋白质相互作用网络研究基因间的相互作用。鉴定了与精子发生相关的通路和基因，包括催产素信号通路、钙信号通路、FOXO信号通路、DNAH家族和其他关键基因。通过qRT-PCR验证了25个关键基因的表达水平。精子发生相关基因的研究结果将为深入了解A.方笑。
Spermatogenesis is the production of spermatozoa by the testes, a process that is associated with reproduction in males.Amphioctopus fangsiaois an economically important species from the northern coast of China, and little research has been done on spermatogenesis in this species. In this study, we performed transcriptome analysis of testis and other somatic tissues. The maturing stage of testis development was selected, in which the emergence of spermatozoa was observed. A total of 15,773 differentially expressed genes were identified, including 8,922 upregulated genes and 6,851 downregulated genes. GO and KEGG enrichment analyses were performed to explore the functions of genes related to spermatogenesis, and the interactions between genes were examined by constructing protein interaction networks. Pathways and genes related to spermatogenesis were identified, including the oxytocin signaling pathway, the calcium signaling pathway, the FOXO signaling pathway, the DNAH family, and other key genes. The expression levels of 25 key genes were verified by qRT-PCR. The results for spermatogenesis-related genes will provide insights into the processes of spermatogenesis and testis development inA. fangsiao.