Separation of 90Nb from zirconium target for application in immuno-PET
Separation of 90Nb from zirconium target for application in immuno-PET
复制标题
从锆靶中分离 90Nb 用于免疫 PET
DOI：
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
F. Roesch
中科院分区：
文献类型：
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作者：
V. Radchenko;D. Filosofov;O. Bochko;N. Lebedev;A. Rakhimov;H. Hauser;M. Eisenhut;N. Aksenov;G. Bozhikov;B. Ponsard;F. Roesch
摘要快速发展的免疫PET要求探索新的放射性核素。最有希望的候选人之一是90 Nb。它的半衰期为14.6小时，允许可视化和定量具有中等和缓慢动力学的生物过程，例如抗体和抗体片段或药物递送系统和纳米颗粒的肿瘤积累。90 Nb显示出53%的正电子分支和发射的正电子的平均动能Emean =0.35 MeV。目前，放射性核素生产路线和NbV标记技术的探索，把这种放射性核素成为一个有用的成像探针。然而，从辐照靶中有效分离90 Nb仍面临挑战。在AG 1 × 8 - HCl/H2 O2和UTEVA-HCl体系中，研究了离子交换法从锆靶中分离90 Nb。选择95 Nb（t1/2 = 35.0 d）、95 Zr（t1/2 = 64.0 d）和92 mNb（t1/2 = 10.15 d）进行分配系数的研究。经AG 1 × 8阴离子交换后分离，90/95 Nb收率达99%。随后在UTEVA树脂上使用固相萃取步骤进一步从痕量锆中净化90/95 Nb，得到95%的90/95 Nb。半自动分离需要一个小时才能获得90%的90/95 Nb总回收率。Zr的量减少了108倍。所选择的分离提供了适用于合成90 Nb-放射性药物的高纯度90 Nb的快速制备（< 1 h），与免疫PET的目的相关。应用获得的放射性铌，用去铁胺修饰的单克隆抗体（利妥昔单抗）的90/95 Nb标记在室温下孵育1小时后实现了>90%的标记产率。
Abstract Fast progressing immuno-PET asks to explore new radionuclides. One of the promising candidates is 90Nb. It has a half-life of 14.6 h that allows visualizing and quantifying biological processes with medium and slow kinetics, such as tumor accumulation of antibodies and antibodies fragments or drug delivery systems and nanoparticles. 90Nb exhibits a positron branching of 53% and an average kinetic energy of emitted positrons of Emean =0.35 MeV. Currently, radionuclide production routes and NbV labeling techniques are explored to turn this radionuclide into a useful imaging probe. However, efficient separation of 90Nb from irradiated targets remains in challenge. Ion exchange based separation of 90Nb from zirconium targets was investigated in systems AG 1 × 8 – HCl/H2O2 and UTEVA-HCl. 95Nb (t1/2 = 35.0 d), 95Zr (t1/2 = 64.0 d) and 92mNb (t1/2 = 10.15 d) were chosen for studies on distribution coefficients. Separation after AG 1 × 8 anion exchange yields 99% of 90/95Nb. Subsequent use of a solid-phase extraction step on UTEVA resin further decontaminates 90/95Nb from traces of zirconium with yields 95% of 90/95Nb. A semi-automated separation takes one hour to obtain an overall recovery of 90/95Nb of 90%. The amount of Zr was reduced by factor of 108. The selected separation provides rapid preparation (< 1 h) of high purity 90Nb appropriate for the synthesis of 90Nb-radiopharmaceuticals, relevant for purposes of immuno-PET. Applying the radioniobium obtained, 90/95Nb-labeling of a monoclonal antibody (rituximab) modified with desferrioxamine achieved labeling yields of >90% after 1 h incubation at room temperature.
影响因子：
4.7
作者：
Zhou, Yang;Liu, Shuang
通讯作者：
Liu, Shuang