Behavioral effects of transient cerebral ischemia.
Behavioral effects of transient cerebral ischemia.
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DOI：
10.1177/109980040000100404
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发表时间：
2000-04-01
影响因子：
2.5
通讯作者：
Therrien, B
中科院分区：
文献类型：
--
作者：
Briones, T L;Therrien, B
海马体中的CA 1神经元是参与学习和记忆的脑结构，选择性地易受缺血效应的影响。在这项研究中，作者研究了缺血持续时间是否与CA 1损伤的程度和空间学习障碍的程度直接相关。成年雌性Wistar大鼠接受5分钟或10分钟的缺血或假手术。恢复后，在Morris水迷宫中测试大鼠。组织学分析显示，在5分钟缺血时，CA 1（31%）和CA 3（12%）中有中度细胞丢失，CA 2（4%）中有极轻微细胞丢失。缺血10分钟时，在CA 1（68%）、CA 2（16%）和CA 3（23%）中观察到细胞丢失增加。行为测试显示，与5分钟缺血组相比，10分钟缺血的动物具有更大的空间学习缺陷，并且在整个测试日它们仍然受损。此外，在缺血组中，CA 1细胞丢失的程度占空间学习缺陷方差的约45%。作者得出结论，在接受5和10分钟缺血的大鼠中，细胞损失主要限于CA 1区域，并且缺血持续时间增加导致雌性大鼠持续性学习障碍;此外，行为障碍的程度与CA 1细胞损失的程度有关。
CA1 neurons in the hippocampus, a brain structure involved in learning and memory, are selectively vulnerable to ischemic effects. In this study, the authors examined if duration of ischemia is directly related to extent of CA1 damage and degree of spatial learning deficit. Adult female Wistar rats received either 5-min or 10-min ischemia or sham surgery. Following recovery, rats were tested in the Morris water maze. Histological analysis showed moderate cell loss in CA1 (31%) and CA3 (12%) and minimal cell loss in CA2 (4%) with 5-min ischemia. Increased cell loss was seen in CA1 (68%), CA2 (16%), and CA3 (23%) with 10-min ischemia. Behavioral testing revealed that animals with 10-min ischemia have greater spatial learning deficits and they remain impaired across the test days compared to the 5-min ischemic group. Furthermore, degree of CA1 cell loss accounted for approximately 45% of the variance in spatial learning deficits in the ischemic group. The authors conclude that cell loss is largely confined to CA1 region in rats who received 5 and 10 min of ischemia and that increased ischemic duration results in persistent learning deficits in female rats; also, the degree of behavioral impairment is related to extent of CA1 cell loss.