Growth and formation of statoliths in Aurelia coerulea examined by using 34S- and Sr-labels
Growth and formation of statoliths in Aurelia coerulea examined by using 34S- and Sr-labels
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使用 34S 和 Sr 标记检查蓝花木中平衡石的生长和形成
DOI：
10.1093/plankt/fby034
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发表时间：
2018
影响因子：
2.1
通讯作者：
Sano Yuji
中科院分区：
文献类型：
--
作者：
Toyokawa Masaya;Shirai Kotaro;Takahata Naoto;Ishida Akizumi;Sano Yuji
本文成功地用~(34)S和~(34)Sr标记了蓝鳍金龟子，并首次用纳米级二次离子质谱仪(NanoSIMS)和电子探针微区分析(EPMA)对其进行了可视化。水母在富含~(34)S和~(34)Sr的人工海水中孵化25小时。用于分析的统计囊固定在两个时间点：暴露后立即(零日)和在非浓缩海水中孵育3周后(孵化后)。在NanoSIMS分析中，观察到在孵化后的样品中，34S以内环的形式被包裹在晶体中。在零日龄标本中，鼻石周边区的34S/32S比值明显高于核心区。在EPMA分析中，零日龄标本中的稳石位于稳囊的底部，有致密的锶沉积。较大的柱状物往往位于距基底部较远的位置。这些发现与稳定期囊底部为稳定期结石起源区的假说相一致。未沉积锶的晶体尺寸往往比有沉积的晶体尺寸大，表明晶体达到一定尺寸后生长达到饱和。
Statoliths ofAurelia coeruleawere successfully labeled with34S and Sr and were visualized for the first time by nanoscale secondary ion mass spectrometry (NanoSIMS) and electron probe microanalysis (EPMA). Medusae were incubated in artificial seawater with an enrichment of34S and Sr for 25 hours. Statocysts for analyses were fixed at two time-points: immediately after the exposure (zero-day) and after 3 weeks of incubation in non-enriched seawater (post-incubated). In the analyses by NanoSIMS, deposition of34S was observed as an inner ring enclosed within the crystal in post-incubated specimens. In zero-day specimens,34S/32S ratio in the peripheral region of statolith was much higher than those in the core region. In the analyses by EPMA, small statoliths with dense deposition of Sr were located in the basal part of statocysts in zero-day specimens. The larger statoliths tended to be located more distant from the basal part. These findings were consistent with the hypothesis that the basal part of statocysts is the area of origin of statoliths. Statoliths without deposition of Sr tend to be larger in size than those with deposition, suggesting the saturation of growth after crystals attain a certain size.