Bone marrow and peripheral blood expression of ID1 in human gastric carcinoma patients is a bona fide indicator of lymph node and peritoneal metastasis.
Bone marrow and peripheral blood expression of ID1 in human gastric carcinoma patients is a bona fide indicator of lymph node and peritoneal metastasis.
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DOI：
10.1038/sj.bjc.6605085
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发表时间：
2009-06-16
影响因子：
8.8
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中科院分区：
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最近的研究表明，骨髓来源的内皮祖细胞在转移中起着关键作用，而ID1作为血管生成的调节因子在转移中是必需的。因此，我们探讨了ID1基因在胃癌患者骨髓和外周血中的表达及其临床意义。收集289例胃癌患者骨髓和外周血标本，采用定量RT-PCR法进行ID1基因检测。采用免疫组织化学方法检测ID1蛋白在1例骨髓、3例转移性淋巴结和3例腹膜播散性肿瘤中的表达。在骨髓和外周血标本中，转移组ID1mRNA的表达均显著高于其他组(P=0.003，P=0.0001)，且与淋巴结转移和腹膜转移显著相关。与健康志愿者相比，转移癌患者骨髓中的细胞ID1染色较强。骨髓和外周血中ID1基因的表达与淋巴结转移和腹膜转移密切相关，可作为预测淋巴结转移和腹膜转移的指标。
Recent studies have showed that the bone marrow-derived endothelial progenitor cells play critical roles in metastasis and that ID1 is required in metastasis as regulator of angiogenesis. Therefore, we investigated the clinical significance of ID1 mRNA expression in bone marrow and peripheral samples in patients with gastric cancer. Two hundred and eighty-nine bone marrow and 196 peripheral blood samples from gastric cancer patients were collected and analysed by quantitative RT–PCR for ID1. The ID1 protein expression in one bone marrow, three metastatic lymph nodes and three peritoneal disseminated tumours was examined by immunohistochemical methods. In both bone marrow and peripheral blood samples, ID1 mRNA expression in the metastatic group was significantly higher than in any other group (P=0.003, P=0.0001, respectively) and significantly associated with lymph node metastasis and peritoneal dissemination. The cells in bone marrow with metastatic cancer stained strongly with ID1 compared with those of healthy volunteers. The expression of ID1 mRNA in bone marrow and peripheral blood was significantly associated with lymph node metastasis and peritoneal dissemination, and therefore constitutes a predictable marker for lymph node metastasis and peritoneal dissemination.