Generalized Bacteriophage Transduction in Serratia marcescens
Generalized Bacteriophage Transduction in Serratia marcescens
复制标题
粘质沙雷氏菌中的广义噬菌体转导
DOI：
10.1007/s12668-016-0268-z
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发表时间：
2016
期刊：
影响因子：
3
通讯作者：
L. Bogomolnaya
中科院分区：
文献类型：
--
作者：
T. Shirshikova;O. Morozova;L. K. Kamaletdinova;M. Sharipova;L. Bogomolnaya
Serratia marcescensis a Gram-negative bacterium from theEnterobacteriaceaefamily. As an opportunistic human pathogen and a causative agent of a range of nosocomial infections,S. marcescensrepresents a growing public health problem. Little is known aboutS. marcescenspathogenicity factors, in part due to the lack of well-developed functional genomic tools. Bacteriophage transduction is one of the main mechanisms of horizontal transfer of DNA in bacteria.S. marcescensbacteriophages previously used in transduction experiments are strain-specific, and no comprehensive studies have previously been done to evaluate the efficiency of transduction across different phages and host strains. In this study, we directly compared the ability of bacteriophages ΦOT8 and ΦIF3 to infect twoS. marcescensstrains, SM6 and SR41-8000. We successfully used bacteriophage ΦOT8 to transfer a mutation intolClocus between twoS. marcescensstrains.
